CAJ | 학술논문

对甘肃省马铃薯（Solanum tuberosum L．）束梗褐腐病病原进行了分离鉴定和生物学特性研究。经形态特征观察与 rDNA-ITS 序列分析，将病原菌鉴定为细基束梗霉[Doratomyces stemonitis （Pers．ex Fr．）F．J．Morton ＆G．Smith]。生物学特性研究结果表明，病菌菌丝生长最适温度为25～30℃，最适 pH 为6，病菌能利用多种碳源，但以蔗糖最好，氮源以甘氨酸最适，光照对菌丝生长没有影响。分生孢子在5～40℃范围内均能萌发，最适25℃，最适 pH 为7，分生孢子萌发需液态水，湿度低于99％几乎不萌发，马铃薯汁液和葡萄糖液对孢子萌发有较好的促进作用。本研究为马铃薯束梗褐腐病的防治提供了理论依据。
대감숙성마령서（Solanum tuberosum L．）속경갈부병병원진행료분리감정화생물학특성연구。경형태특정관찰여 rDNA-ITS 서렬분석，장병원균감정위세기속경매[Doratomyces stemonitis （Pers．ex Fr．）F．J．Morton ＆G．Smith]。생물학특성연구결과표명，병균균사생장최괄온도위25～30℃，최괄 pH 위6，병균능이용다충탄원，단이자당최호，담원이감안산최괄，광조대균사생장몰유영향。분생포자재5～40℃범위내균능맹발，최괄25℃，최괄 pH 위7，분생포자맹발수액태수，습도저우99％궤호불맹발，마령서즙액화포도당액대포자맹발유교호적촉진작용。본연구위마령서속경갈부병적방치제공료이론의거。
The identification and biological characteristics of the pathogen isolated from brown rot of Solanum tu-berosum were studied.The pathogen was identified as Doratomyces stemonitis (Pers.ex Fr.)F.J.Morton &G. Smith based on morphological characteristics as well as rDNA-ITS sequences.The optimum temperature for myce-lial growth was 25-30 ℃ at pH 6.The pathogen could use monosaccharide,disaccharide and polysaccharide;the best carbon source was sucrose,and the best nitrogen source was glycine.Illumination had no obvious promotion effect on mycelial growth.The optimum temperature for sporangium sprout was 25 ℃ at pH 7.Spores could ger-minate only in water drop.The filtrate of potato and glucose solution had obvious promotion effect on spore ger-mination.Soil solution had no effects on spore germination.