中国保健营养(中旬刊)
中國保健營養(中旬刊)
중국보건영양(중순간)
China Hwalth Care & nutrition
2013年
7期
858-860
,共3页
董历明%唐仁权%王琬婷%冯照强%陈玉%王亚鹏%李杨
董歷明%唐仁權%王琬婷%馮照彊%陳玉%王亞鵬%李楊
동력명%당인권%왕완정%풍조강%진옥%왕아붕%리양
真菌%原虫%白血病
真菌%原蟲%白血病
진균%원충%백혈병
Fungi%protozoa%leukemia
目的:检测各类白血病患者静脉血中感染的真菌和原虫的种类和区别,寻求敏感药物治疗。方法:白血病患者静脉血50-100μl滴加在1640和生理盐水混合液中培养,22-36度条件下,置100×显微镜下观察接种后至120小时的真菌和原虫生长感染红细胞状况。结果:9种不同类型白血病患者静脉血培养中镜下均可见同一种①真菌生长,其中4种可见原虫伴随生长,5种见另一种②真菌混合生长。当有混合微生物生长时,镜下观察,伴随温度的提高,①真菌在红细胞内繁殖速度加快,二分裂倍数增长,最后呈喷射状释放到红细胞体外,呈球形游离单体,运动速度极快,致使血红蛋白流失,形成影形红细胞,导致患者贫血。温度低时,即结成杆状菊花状。同时可见血小板被①真菌侵袭后运动速度加快,形成影形印戒形,被白细胞吞噬。结论:9类白血病患者静脉血中见到同一类真菌可为正常人类红细胞中感染的一种真菌,当伴有其他原虫和真菌类病原微生物侵袭时诱发骨髓内幼稚型白细胞释放入血,引发白血病。
目的:檢測各類白血病患者靜脈血中感染的真菌和原蟲的種類和區彆,尋求敏感藥物治療。方法:白血病患者靜脈血50-100μl滴加在1640和生理鹽水混閤液中培養,22-36度條件下,置100×顯微鏡下觀察接種後至120小時的真菌和原蟲生長感染紅細胞狀況。結果:9種不同類型白血病患者靜脈血培養中鏡下均可見同一種①真菌生長,其中4種可見原蟲伴隨生長,5種見另一種②真菌混閤生長。噹有混閤微生物生長時,鏡下觀察,伴隨溫度的提高,①真菌在紅細胞內繁殖速度加快,二分裂倍數增長,最後呈噴射狀釋放到紅細胞體外,呈毬形遊離單體,運動速度極快,緻使血紅蛋白流失,形成影形紅細胞,導緻患者貧血。溫度低時,即結成桿狀菊花狀。同時可見血小闆被①真菌侵襲後運動速度加快,形成影形印戒形,被白細胞吞噬。結論:9類白血病患者靜脈血中見到同一類真菌可為正常人類紅細胞中感染的一種真菌,噹伴有其他原蟲和真菌類病原微生物侵襲時誘髮骨髓內幼稚型白細胞釋放入血,引髮白血病。
목적:검측각류백혈병환자정맥혈중감염적진균화원충적충류화구별,심구민감약물치료。방법:백혈병환자정맥혈50-100μl적가재1640화생리염수혼합액중배양,22-36도조건하,치100×현미경하관찰접충후지120소시적진균화원충생장감염홍세포상황。결과:9충불동류형백혈병환자정맥혈배양중경하균가견동일충①진균생장,기중4충가견원충반수생장,5충견령일충②진균혼합생장。당유혼합미생물생장시,경하관찰,반수온도적제고,①진균재홍세포내번식속도가쾌,이분렬배수증장,최후정분사상석방도홍세포체외,정구형유리단체,운동속도겁쾌,치사혈홍단백류실,형성영형홍세포,도치환자빈혈。온도저시,즉결성간상국화상。동시가견혈소판피①진균침습후운동속도가쾌,형성영형인계형,피백세포탄서。결론:9류백혈병환자정맥혈중견도동일류진균가위정상인류홍세포중감염적일충진균,당반유기타원충화진균류병원미생물침습시유발골수내유치형백세포석방입혈,인발백혈병。
Objective:In the present study, in order to find sensitive to drug treatment, we tested difference of fungi and protozoan in blood in various types of leukemia in patients. Methods:Under 22-36℃, 50-100μl blood of leukemia patients cultured in solution of 1640 and saline, and observed fungi and protozoa infected red cells growth condition under 100 × microscope after vaccination to 120 hours. Results:9 different types of leukemia patients are visible with the same kind of fungalⅠgrowth in venous blood, 4 of them accompanied growth with protozoa, 5 of them mixed growth with another fungal I . With high temperature, the fungusⅠinside red blood cellpropagation speeded, two split multiply, sprayed to the extracellular which moved speedily like spherical free monomer. Red blood celllost hemoglobin and formed shadow shaped, final y it induced anemia. With low temperature, fungalⅠformed rod-shaped chrysanthemum. After infected fungalⅠ, platelets moved faster, formed a film-shaped signet-ring shaped, final y were phagocytosed by leukocytes. Conclusion: 9 different types of leukemia patients were visible with the same kind of fungalⅠ growth in venous blood. When accompanied by other protozoa and fungi invasion, it induced juvenile white cells released into the blood, caused leukemia.