CAJ | 학술논문

目的：研究Pim-1基因及其蛋白在宫颈鳞癌组织中的表达水平，探讨其与宫颈鳞癌临床特性之间的关系。方法：采用逆转录-聚合酶链反应（RT-PCR）半定量方法和免疫组化学法，检测46例宫颈鳞癌组织和10例正常宫颈组织标本中Pim-1基因及蛋白的表达情况。结果：宫颈鳞癌组织中Pim-1基因与蛋白的表达水平明显高于宫颈正常组织（P<0.05）；Pim-1蛋白在宫颈鳞癌组织中的表达与组织学分级、临床分期和淋巴结转移密切相关（P<0.05）。结论：原癌基因Pim-1及其蛋白在宫颈鳞癌组织中过度表达，可能与宫颈鳞癌的发生、发展有关。
목적：연구Pim-1기인급기단백재궁경린암조직중적표체수평，탐토기여궁경린암림상특성지간적관계。방법：채용역전록-취합매련반응（RT-PCR）반정량방법화면역조화학법，검측46례궁경린암조직화10례정상궁경조직표본중Pim-1기인급단백적표체정황。결과：궁경린암조직중Pim-1기인여단백적표체수평명현고우궁경정상조직（P<0.05）；Pim-1단백재궁경린암조직중적표체여조직학분급、림상분기화림파결전이밀절상관（P<0.05）。결론：원암기인Pim-1급기단백재궁경린암조직중과도표체，가능여궁경린암적발생、발전유관。
Objective:To study the gene and protein expressions of Pim-1 in human cervical squamous cell carcinoma tissues,and to analyze the relationship between expressions and clinical characteristics. Method:Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry analysis were used to detect the gene and protein expressions of Pim-1 in 46 cervical squamous cell carcinoma tissues and 10 normal cervical tissues;and their relations with clinical characteristics were analyzed. Result:The gene and protein expressions of Pim-1 in cervical squamous cell carcinoma tissues were significantly higher than that in the normal cervical tissues(P<0.05);The protein expressions of Pim-1 in human cervical squamous cell carcinoma tissues were significantly related with pathologic grade,FIGO stage and lymph node metastasis of cervical squamous cell carcinoma(P<0.05). Conclusion:Pro-oncogene Pim-1 and their protein are aberrant expressed in human cervical squamous cell carcinoma tissues, and they may be important for the occurrence and development of cervical squamous cell carcinoma.