CAJ | 학술논문

目的：探讨实时荧光定量PCR对乙肝DNA检测的效果，对影响因素进行分析总结。方法：选择2010年1月-2012年1月笔者所在医院收治的300例乙肝病毒感染患者，收集其血标本，通过实时荧光定量PCR检测方法进行乙肝DNA检验，对检验结果与临床诊断结果进行对比分析，对影响检验结果的因素进行分析总结。结果：本组300例乙肝感染患者血标本中，采用实时荧光定量PCR对乙肝DNA检测结果与临床诊断结果相同276例(92.0%)，漏诊误诊24例(8.0%)，主要的影响因素包括实验室因素、标本因素和核酸提取方法因素等。结论：采用实时荧光定量PCR对乙肝DNA进行检测，具有较高的确诊率，但对检测结果产生影响的因素较多，需要加强注意，才能降低漏诊和误诊率，为治疗提供有效支持。
목적：탐토실시형광정량PCR대을간DNA검측적효과，대영향인소진행분석총결。방법：선택2010년1월-2012년1월필자소재의원수치적300례을간병독감염환자，수집기혈표본，통과실시형광정량PCR검측방법진행을간DNA검험，대검험결과여림상진단결과진행대비분석，대영향검험결과적인소진행분석총결。결과：본조300례을간감염환자혈표본중，채용실시형광정량PCR대을간DNA검측결과여림상진단결과상동276례(92.0%)，루진오진24례(8.0%)，주요적영향인소포괄실험실인소、표본인소화핵산제취방법인소등。결론：채용실시형광정량PCR대을간DNA진행검측，구유교고적학진솔，단대검측결과산생영향적인소교다，수요가강주의，재능강저루진화오진솔，위치료제공유효지지。
Objective:To explore effect of real-time fluorescent quantitative PCR detection of hepatitis B DNA,analyze the influence factors.Method:From January 2010 to January 2012 were between 300 patients with hepatitis b virus (HBV) infection,collect the blood specimens,by real-time fluorescent quantitative PCR detection method for hepatitis B DNA testing,the result of the inspection result and clinical diagnosis,this paper compares and analyzes the effect factors of test results is analyzed.Result:300 cases of hepatitis b infection in patients with blood specimens,using real-time fluorescent quantitative PCR of hepatitis B DNA test results and clinical diagnosis results of 276 cases of same (92.0%),misdiagnosis and misdiagnosed 24 cases,accounted for 8.0%,the main influence factors including laboratories,specimen and nucleic acid extraction method,etc.Conclusion:The real-time fluorescent quantitative PCR to detect HBV DNA,has higher diagnosis rate,but the influence on test results of many factors,the need to strengthen the attention,to decrease the rate of missed diagnosis and misdiagnosis,provide effective support for treatment.