CAJ | 학술논문

通过动态光散射技术与原子力显微镜观察了Co（NH3）3＋6导致的λ-DNA凝聚。用动态光散射测量溶液中Co（NH3）3＋6作用后的DNA粒径大小，结果表明凝聚后的粒径与样品培育时间和Co（NH3）3＋6的浓度有关，随着培育时间或者Co （N H3）3＋6浓度的增加，凝聚后的粒径都会慢慢变小，最后趋于稳定。粒径的分析采用单峰的分布模式处理，表明所有DNA由松散构象向凝聚构象转化的过程基本趋于一致。用原子力显微镜观察Co（NH3）3＋6导致DNA凝聚的形态变化，并进一步证明了凝聚粒径的单峰分布模式。
통과동태광산사기술여원자력현미경관찰료Co（NH3）3＋6도치적λ-DNA응취。용동태광산사측량용액중Co（NH3）3＋6작용후적DNA립경대소，결과표명응취후적립경여양품배육시간화Co（NH3）3＋6적농도유관，수착배육시간혹자Co （N H3）3＋6농도적증가，응취후적립경도회만만변소，최후추우은정。립경적분석채용단봉적분포모식처리，표명소유DNA유송산구상향응취구상전화적과정기본추우일치。용원자력현미경관찰Co（NH3）3＋6도치DNA응취적형태변화，병진일보증명료응취립경적단봉분포모식。
The condensation ofλ-DNA by Co(NH3 )3+6 was investigated with dynamic light scatter-ing (DLS) and atom force microscope (AFM) .The hydrodynamic radius of DNA-Co(NH3 )3+6 comple-xes in solution was measured by DLS .The results showed that the particle sizes of the complex de-pend on the incubation time of the sample and the concentration of the condensing agent .Specifically , the sizes of the condensed particles decreased with the incubation time and Co(NH3 )3+6 concentration and finally approached to stable values .The particle sizes were analyzed with single-peak distribution model ,i .e .,the changes of all DNA from coil form to condensed structures underwent the same con-densing process .The condensed morphologies of DNA induced by Co(NH3 )3+6 were observed by AFM and it affirmed the single-peak distribution model of the particle sizes in DLS .