CAJ | 학술논문

目的：评价肺炎支原体快速培养法在小儿肺炎支原体(MP)肺炎诊断中的应用价值。方法：采用咽拭子MP快速培养法和ELISA半定量检测血清MP-IgM抗体同时检测248例呼吸道感染小儿患者，对检查结果进行统计学分析。结果：248例患儿中,快速培养法检出21例阳性,阳性率22.98%；ELISA法检出MP-IgM阳性25例，阳性率27.42%，两种方法检测阳性检出数差异有统计学意义(P=0.000)；两种方法一致性较好(kappa=0.819>0.75， T=12.982，P=0.000)；以临床诊断结果评价两种实验室检测方法绘制ROC曲线，AUC快速培养=0.880，AUC血清MP-IgM=0.820。结论：MP快速培养快速简便，较血清MP-IgM检测更适用于早期和免疫系统尚未发育完善及免疫缺陷患者的诊断，与血清MP-IgM检测联合应用可提高MP的诊断效率。
목적：평개폐염지원체쾌속배양법재소인폐염지원체(MP)폐염진단중적응용개치。방법：채용인식자MP쾌속배양법화ELISA반정량검측혈청MP-IgM항체동시검측248례호흡도감염소인환자，대검사결과진행통계학분석。결과：248례환인중,쾌속배양법검출21례양성,양성솔22.98%；ELISA법검출MP-IgM양성25례，양성솔27.42%，량충방법검측양성검출수차이유통계학의의(P=0.000)；량충방법일치성교호(kappa=0.819>0.75， T=12.982，P=0.000)；이림상진단결과평개량충실험실검측방법회제ROC곡선，AUC쾌속배양=0.880，AUC혈청MP-IgM=0.820。결론：MP쾌속배양쾌속간편，교혈청MP-IgM검측경괄용우조기화면역계통상미발육완선급면역결함환자적진단，여혈청MP-IgM검측연합응용가제고MP적진단효솔。
Objective To evaluation the Mycoplasma pneumoniae rapid culture method in detection of my-coplasma pneumonia (MP) application value. Methods Using the throat swab MP rapid culture assay for simulta-neous detection and ELISA method for quantitative of serum MP-IgM antibodies detection of 248 cases of respi-ratory tract infections in pediatric patients , the test was used for statistical analysis. Results In all the 248 cases, rapid culture assay for detection of 21 positive cases, the positive rate of 22.98%;ELISA method for detection of MP-IgM was positive in 25 cases, the positive rate was 27.42%, two kinds of methods for detection of the positive number of MP had significant difference (P=0.000). two kinds of methodshad better consistency (kappa=0.819>0.75,T=12.982,P=0.000);Using the results of clinical diagnosis to evaluatetwo kinds of laboratory testing methods and draw the ROC curve, AUCrapid culture=0.880,AUCserum MP-IgM=0.820. Conclusion MP rapid culture is a quick and convenientmethod, especially in the early, immune system not yet mature and immunodeficiencypa-tients is better thanserum MP-IgM,can improve the diagnosis efficiency by combined with serum MP-IgM.