CAJ | 학술논문

目的：研究携带吲哚胺2，3-二氧化酶（IDO）的小鼠肝细胞在同种异体脾内的表达及对脾淋巴细胞的凋亡作用。方法分离BABL/c小鼠肝细胞，肝细胞脾内移植（BABL/c→C57BL/6），分为4组：Ⅰ组为携带IDO组；Ⅱ组为1-甲基色氨酸组；Ⅲ组为空壳腺病毒组；Ⅳ组为单纯肝细胞移植组。移植术后第2，5d取脾脏免疫组织化学检测IDO表达情况，TUNEL检测脾脏淋巴细胞凋亡情况。结果携带IDO腺病毒转染肝细胞后，能有效表达IDO。免疫组织化学显示，Ⅰ、Ⅱ组脾脏有IDO阳性的肝细胞。与其他组比较，移植术后第2，5dⅠ组淋巴细胞凋亡差别有统计学意义（P＜0．05），Ⅱ、Ⅲ、Ⅳ组之间比较，差别无统计学意义（P＞0．05）。结论携带IDO的肝细胞能在小鼠同种异体脾内稳定表达，并能诱导脾内淋巴细胞的凋亡。
목적：연구휴대신타알2，3-이양화매（IDO）적소서간세포재동충이체비내적표체급대비림파세포적조망작용。방법분리BABL/c소서간세포，간세포비내이식（BABL/c→C57BL/6），분위4조：Ⅰ조위휴대IDO조；Ⅱ조위1-갑기색안산조；Ⅲ조위공각선병독조；Ⅳ조위단순간세포이식조。이식술후제2，5d취비장면역조직화학검측IDO표체정황，TUNEL검측비장림파세포조망정황。결과휴대IDO선병독전염간세포후，능유효표체IDO。면역조직화학현시，Ⅰ、Ⅱ조비장유IDO양성적간세포。여기타조비교，이식술후제2，5dⅠ조림파세포조망차별유통계학의의（P＜0．05），Ⅱ、Ⅲ、Ⅳ조지간비교，차별무통계학의의（P＞0．05）。결론휴대IDO적간세포능재소서동충이체비내은정표체，병능유도비내림파세포적조망。
Objective To evaluate the effects of Indoleamine 2 ,3-dioxygenase (IDO) on the mice allogeneic hepatocytes transplanted into spleen . Methods BABL/c mice hepatocytes were isolated and transplanted into C57BL/6 mouse and divided into IDO group (I group ) , IDO + 1-methyl tryptophan (1-MT) group(Ⅱ group) ,shell adenovirus group(Ⅲ group) and hepatocyte group(Ⅳ group) . The IDO was detected by immunohistochemistry and lymphocyte apoptosis of spleen were detected by TUNEL on the day 2 ,5 after transplantation . Results Hepatocytes effectively expressed IDO after transfection . Immunohistochemistry showed that IDO-positive hepatocytes were observed in Ⅰ and Ⅱ group . Com-pared with other groups ,the difference of lymphocyte apoptosis in Ⅰ group was statistically significant on the day 2 ,5 after transplantation(P<0 .05) ,no difference were observed between Ⅱ ,Ⅲ and Ⅳ groups (P>0 .05) . Conclusion Mice allogeneic hepatocytes transfected with IDO can stably express IDO and induce lymphocyte apoptosis in spleen .