CAJ | 학술논문

目的：探讨Nicotiflorin（Kaempferol 3-O-β-rutinoside ，山奈酚-3-O-芸香糖苷）对人乳腺癌MCF-7细胞的增殖抑制，以及诱导细胞凋亡和对细胞周期的影响。方法采用M T S法检测细胞存活率，计算IC50值；流式细胞仪检测细胞周期及细胞凋亡；Western-blot检测DNA损伤标记蛋白γ-H2AX及凋亡相关蛋白Bcl-2的变化。结果 Nicotiflorin能剂量依赖性地抑制人乳腺癌MCF-7细胞的增殖，IC50值为28．2μmol/L。流式细胞仪检测结果显示，Nicotiflorin使MCF-7细胞周期阻滞于G2/M 期，不同浓度的Nicotiflorin作用48 h后细胞凋亡率均增加。Western-blot检测发现，Nicotiflorin处理后，磷酸化的 H2AX（γ-H2AX）随时间依赖性地增加，提示 Nicoti-florin诱导DNA双链断裂从而使细胞阻滞于G2/M期，同时Nicotiflorin可能通过抑制抗凋亡蛋白Bcl-2的表达诱导细胞凋亡。结论 Nicotiflorin能抑制人乳腺癌MCF-7细胞的增殖并诱导其凋亡。
목적：탐토Nicotiflorin（Kaempferol 3-O-β-rutinoside ，산내분-3-O-예향당감）대인유선암MCF-7세포적증식억제，이급유도세포조망화대세포주기적영향。방법채용M T S법검측세포존활솔，계산IC50치；류식세포의검측세포주기급세포조망；Western-blot검측DNA손상표기단백γ-H2AX급조망상관단백Bcl-2적변화。결과 Nicotiflorin능제량의뢰성지억제인유선암MCF-7세포적증식，IC50치위28．2μmol/L。류식세포의검측결과현시，Nicotiflorin사MCF-7세포주기조체우G2/M 기，불동농도적Nicotiflorin작용48 h후세포조망솔균증가。Western-blot검측발현，Nicotiflorin처리후，린산화적 H2AX（γ-H2AX）수시간의뢰성지증가，제시 Nicoti-florin유도DNA쌍련단렬종이사세포조체우G2/M기，동시Nicotiflorin가능통과억제항조망단백Bcl-2적표체유도세포조망。결론 Nicotiflorin능억제인유선암MCF-7세포적증식병유도기조망。
Objective To study the effect of Nicotiflorin (Kaempferol 3-O-β-rutinoside) on prolif-eration ,cell cycle distribution and apoptosis of human breast cancer cells MCF-7 . Methods M TS assay was performed to detect the viability of MCF-7 cells and flow cytometry were used to observe the effect of Nicotiflorin on cell cycle distribution and apoptosis . Western blot was applied to deteted γ-H2AX at the DNA double-strand breaks (DSBs) of chromatin as well as Bcl-2 expression . Result Nicotiflorin inhibi-ted the proliferation of MCF-7 cells in a dose-dependent manner and IC50 is 28 .2 μmol/L . Nicotiflorin arrested MCF-7 cells at G2/M phase and induced apoptosis . Western blot showed that Nicotiflorin in-duced DSBs and dow n-regulated the expression of Bcl-2 in a time-dependent manner . Conclusion Nicoti-florin has the biological activity to inhibit proliferation ,cell cycle of human breast cancer MCF-7 cell as well as to induce cell apoptosis .