CAJ | 학술논문

采集鱼样品25批，利用PCR检测方法对我国福建部分沿海地区真鲷虹彩病毒病（RSIVD）情况进行初步调查，对调查阳性结果的 PCR扩增片段和阳性病毒株 MCP基因 PCR扩增片段进行测序分析，同时，与GenBank的虹彩病毒科各个属MCP全基因代表株进行同源性比较。初步调查结果表明，我国福建部分沿海地区共检出2批RSIVD阳性样品，受感染鱼种为大黄鱼和斜带髭鲷，将获得的大黄鱼源虹彩病毒、斜带髭鲷虹彩病毒分别命名为RISV-FJ-DHY 和RISV-FJ-XDZD ；通过基因全序列比对和绘制同源树，结果显示，分离的2例病毒株均属于虹彩病毒科细胞肿大病毒属， RISV-FJ-DHY与2002年分离的大黄鱼病毒株发生了一定的变异，而RISV-FJ-XDZD遗传性状稳定。
채집어양품25비，이용PCR검측방법대아국복건부분연해지구진조홍채병독병（RSIVD）정황진행초보조사，대조사양성결과적 PCR확증편단화양성병독주 MCP기인 PCR확증편단진행측서분석，동시，여GenBank적홍채병독과각개속MCP전기인대표주진행동원성비교。초보조사결과표명，아국복건부분연해지구공검출2비RSIVD양성양품，수감염어충위대황어화사대자조，장획득적대황어원홍채병독、사대자조홍채병독분별명명위RISV-FJ-DHY 화RISV-FJ-XDZD ；통과기인전서렬비대화회제동원수，결과현시，분리적2례병독주균속우홍채병독과세포종대병독속， RISV-FJ-DHY여2002년분리적대황어병독주발생료일정적변이，이RISV-FJ-XDZD유전성상은정。
To investigate the infection of red sea bream iridoviral disease (RSIVD) ,25 batches of fish collected from coasta1 areas of Fujian Province were examined by PCR method ,the positive amplified fragments and MCP gene of positive virus strains were sequenced and matched with the reported Iridoviridae MCP gene in Genbank .The result showed that two RSIVD strains was detected in samples from large yellow croaker and hapalogenys nitens respectively ,which were named RISV-FJ-DHY and RISV-FJ-XDZD and found belonging to Megalocytivirus genus of Iridoviridae .Mutations were found in RISV-FJ-DHY and the strains isolated from large yellow croaker in 2002 , while RISV-FJ-XDZD was genetically stable .