CAJ | 학술논문

为提高重组大肠杆菌表达天蚕素AD和BuforinⅡ融合蛋白的表达效率，研究表达菌株、质粒稳定性和诱导条件对天蚕素AD和BuforinⅡ融合蛋白表达的影响。通过SDS-PAGE分析目的蛋白占全菌总蛋白的含量，确定大肠杆菌BER2566为表达菌株；利用基因工程技术，构建卡那霉素抗性的表达质粒pET （K ）-T rx-CAD-BuforinII ，提高质粒稳定性和蛋白表达效率；优化了IPTG浓度、诱导时机和诱导时间等诱导条件，确定菌体浓度为OD600＝0.8时， IPTG浓度为0.8 mmol · L -1，诱导时间为5 h ，目的蛋白表达量最高，可达到全菌总蛋白含量的50％以上。
위제고중조대장간균표체천잠소AD화BuforinⅡ융합단백적표체효솔，연구표체균주、질립은정성화유도조건대천잠소AD화BuforinⅡ융합단백표체적영향。통과SDS-PAGE분석목적단백점전균총단백적함량，학정대장간균BER2566위표체균주；이용기인공정기술，구건잡나매소항성적표체질립pET （K ）-T rx-CAD-BuforinII ，제고질립은정성화단백표체효솔；우화료IPTG농도、유도시궤화유도시간등유도조건，학정균체농도위OD600＝0.8시， IPTG농도위0.8 mmol · L -1，유도시간위5 h ，목적단백표체량최고，가체도전균총단백함량적50％이상。
Effects of expression strains ,plasmid stability and inducing conditions were studied on improving the expression efficiency of Cecropin AD and Buforin II fusion protein in recombinant Escherichia coli .According to the result of SDS-PAGE ,E .coli BER2566 was accepted as expression strain .Plasmid pET (K)-Trx-CAD-BuforinII with kanamycin resistant gene was constructed to improve the plasmid stability and protein expression .Inducing conditions including IPTG concentration ,induction time and inducing time length were optimized .With the optimal conditions ,the highest expression level of fusion protein was obtained in recombinant E .coli at the level of OD600 0.8 and final IPTG concentration of 0.8 mmol · L -1 for 5h inducing ,which was more than 50% of the total protein in E .coli .