福建农业学报
福建農業學報
복건농업학보
FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
2013年
12期
1267-1272
,共6页
许泳清%李华伟%刘中华%邱永祥%罗文彬%纪荣昌%汤浩%邱思鑫%余华
許泳清%李華偉%劉中華%邱永祥%囉文彬%紀榮昌%湯浩%邱思鑫%餘華
허영청%리화위%류중화%구영상%라문빈%기영창%탕호%구사흠%여화
甘薯%病毒%甘薯羽状斑驳病毒%ELISA%RT-PCR
甘藷%病毒%甘藷羽狀斑駁病毒%ELISA%RT-PCR
감서%병독%감서우상반박병독%ELISA%RT-PCR
sweet potato%virus%sweet potato feathery mottle virus%ELISA%RT-PCR
利用双抗体酶联免疫吸附测定法（DAS-ELISA ）对采自福建省51份甘薯叶片进行检测，结果显示51份样品中有24份SPFM V呈阳性，阳性率为47.06％，其中泉州样品SPFM V阳性率最高，为71.40％，其次为南安和龙岩的样品，阳性率均为50％。根据GenBank中公布的SPFM V外壳蛋白（CP）基因序列保守区域设计了1对特异性引物，通过RT-PCR反应程序的优化建立了能检测SPFM V的检测方法。该检测方法能够扩增出SPFM V特异性片段，片段大小为441 bp ，测序结果表明， SPFM V序列与参考序列的同源性92％～97％。
利用雙抗體酶聯免疫吸附測定法（DAS-ELISA ）對採自福建省51份甘藷葉片進行檢測，結果顯示51份樣品中有24份SPFM V呈暘性，暘性率為47.06％，其中泉州樣品SPFM V暘性率最高，為71.40％，其次為南安和龍巖的樣品，暘性率均為50％。根據GenBank中公佈的SPFM V外殼蛋白（CP）基因序列保守區域設計瞭1對特異性引物，通過RT-PCR反應程序的優化建立瞭能檢測SPFM V的檢測方法。該檢測方法能夠擴增齣SPFM V特異性片段，片段大小為441 bp ，測序結果錶明， SPFM V序列與參攷序列的同源性92％～97％。
이용쌍항체매련면역흡부측정법（DAS-ELISA ）대채자복건성51빈감서협편진행검측，결과현시51빈양품중유24빈SPFM V정양성，양성솔위47.06％，기중천주양품SPFM V양성솔최고，위71.40％，기차위남안화룡암적양품，양성솔균위50％。근거GenBank중공포적SPFM V외각단백（CP）기인서렬보수구역설계료1대특이성인물，통과RT-PCR반응정서적우화건립료능검측SPFM V적검측방법。해검측방법능구확증출SPFM V특이성편단，편단대소위441 bp ，측서결과표명， SPFM V서렬여삼고서렬적동원성92％～97％。
51 samples of sweet potato from Fujian province were detected by the double antibody enzyme-linked immunosorbent method (DAS-ELISA) . The results showed that 24 samples were SPFMV-positive ,the positive rate were 47.06% ;the highest positive rate was 71.40% ,detected in the samples from Quanzhou ,and the next were 50.00% ,detected in samples from Nanan and Longyan .A pair of primer was designed and synthesized according to the published nucleotide sequence of coat protein gene of SPFMV ,and a specific RT-PCR detection procedure was established .The amplified SPFMV-specific fragment in PCR contained 441bps ,and showed 92% -97% ,homology with the SPFMV reference sequence .
