CAJ | 학술논문

通过果蝇生存试验研究葡萄柚果汁的体内抗氧化功效和延缓衰老作用。在果蝇培养基中分别加入5％、10％、20％的葡萄柚果汁，测定果蝇平均寿命和最高寿命；并测定果蝇组织匀浆中的超氧化物歧化酶（SOD ）值和丙二醛（MDA ）含量。结果显示葡萄柚果汁低、中、高剂量组对雄果蝇平均寿命的延长率分别为8.24％、20.06％和13.50％，对雌果蝇平均寿命的延长经分别为12.04％、24.93％和19.33％，差异均达极显著水平。不同浓度的葡萄柚果汁均能提高雄、雌果蝇体内的SOD活性，降低MDA含量水平。其中添加10％果汁效果最好，与果蝇生存试验结果一致。
통과과승생존시험연구포도유과즙적체내항양화공효화연완쇠로작용。재과승배양기중분별가입5％、10％、20％적포도유과즙，측정과승평균수명화최고수명；병측정과승조직균장중적초양화물기화매（SOD ）치화병이철（MDA ）함량。결과현시포도유과즙저、중、고제량조대웅과승평균수명적연장솔분별위8.24％、20.06％화13.50％，대자과승평균수명적연장경분별위12.04％、24.93％화19.33％，차이균체겁현저수평。불동농도적포도유과즙균능제고웅、자과승체내적SOD활성，강저MDA함량수평。기중첨가10％과즙효과최호，여과승생존시험결과일치。
Using fruit fly (Drosophila melanogaster) survival experiment ,we investigated the antioxidant and anti-aging functions of grapefruit juice in vitro .Low dose (5% )、middle dose (10% ) and high dose (20% ) of grapefruit juice were added into the basic medium for fruit fly respectively ,the average lifespan and the highest lifespan of the fruit flies fed in three groups were calculated .Moreover ,SOD activity and MDA content in the homogenate of fruit fly body were determined . The results showed that , with low , middle and high dosages , the grapefruit juice significantly prolonged the average lifespan of male fruit flies by 8.24% ,20.06% and 13.50% ,respectively ,while 12.04% ,24.93% and 19.33% for it of the female fruit flies , respectively . Feeding grapefruit juice in certain concentrate could improve SOD activity and reduce MDA content for both male and female fruit flies .The data from middle dose group showed the best effects which coincide with the results of fruit fly survival experiment .