现代诊断与治疗
現代診斷與治療
현대진단여치료
MODERN DIAGNOSIS AND TREATMENT
2013年
18期
4083-4086
,共4页
霍大浪%宁家辉%张袁媛%冯成军%江齐林%刘代顺
霍大浪%寧傢輝%張袁媛%馮成軍%江齊林%劉代順
곽대랑%저가휘%장원원%풍성군%강제림%류대순
BMSC上清%大鼠%急性肝衰竭
BMSC上清%大鼠%急性肝衰竭
BMSC상청%대서%급성간쇠갈
BMSC supernatant%Treatment%Acute liver failure
目的：探讨骨髓间充质干细胞（BMSC）上清治疗大鼠急性肝衰竭（ALF）的疗效，为其临床应用提供理论依据。方法采用全骨髓贴壁筛选法培养SD大鼠BMSC并收集其上清。腹腔注射D-氨基半乳糖胺（D-gal）建立大鼠ALF模型；将造模成功的大鼠随机分为治疗组和对照组，分别将等体积的BMSC上清及0.9%氯化钠注射液（NS）腹腔注射治疗3d，比较两组动物的肝功能、肝组织病理改变和7d生存率。结果（1）7d存活率分别为：BMSC上清治疗组70%，对照组20%，有显著差异（P＜0.05）。（2）BMSC上清治疗组的ALT、AST、TBIL明显降低，而对照组肝功能损害无明显改变，二者比较有显著性差异（P<0.01）。（3）BMSC上清治疗组肝脏病理组织学损害明显改善。结论 BMSC上清腹腔注射治疗可明显改善ALF大鼠的肝功能及肝脏组织学损害，提高ALF大鼠的存活率，为ALF的治疗提供了新的途径。
目的：探討骨髓間充質榦細胞（BMSC）上清治療大鼠急性肝衰竭（ALF）的療效，為其臨床應用提供理論依據。方法採用全骨髓貼壁篩選法培養SD大鼠BMSC併收集其上清。腹腔註射D-氨基半乳糖胺（D-gal）建立大鼠ALF模型；將造模成功的大鼠隨機分為治療組和對照組，分彆將等體積的BMSC上清及0.9%氯化鈉註射液（NS）腹腔註射治療3d，比較兩組動物的肝功能、肝組織病理改變和7d生存率。結果（1）7d存活率分彆為：BMSC上清治療組70%，對照組20%，有顯著差異（P＜0.05）。（2）BMSC上清治療組的ALT、AST、TBIL明顯降低，而對照組肝功能損害無明顯改變，二者比較有顯著性差異（P<0.01）。（3）BMSC上清治療組肝髒病理組織學損害明顯改善。結論 BMSC上清腹腔註射治療可明顯改善ALF大鼠的肝功能及肝髒組織學損害，提高ALF大鼠的存活率，為ALF的治療提供瞭新的途徑。
목적：탐토골수간충질간세포（BMSC）상청치료대서급성간쇠갈（ALF）적료효，위기림상응용제공이론의거。방법채용전골수첩벽사선법배양SD대서BMSC병수집기상청。복강주사D-안기반유당알（D-gal）건립대서ALF모형；장조모성공적대서수궤분위치료조화대조조，분별장등체적적BMSC상청급0.9%록화납주사액（NS）복강주사치료3d，비교량조동물적간공능、간조직병리개변화7d생존솔。결과（1）7d존활솔분별위：BMSC상청치료조70%，대조조20%，유현저차이（P＜0.05）。（2）BMSC상청치료조적ALT、AST、TBIL명현강저，이대조조간공능손해무명현개변，이자비교유현저성차이（P<0.01）。（3）BMSC상청치료조간장병리조직학손해명현개선。결론 BMSC상청복강주사치료가명현개선ALF대서적간공능급간장조직학손해，제고ALF대서적존활솔，위ALF적치료제공료신적도경。
Objective To investigate the bone marrow mesenchymal stem cells(BMSC)supernatant tre atment of acute liver failure (ALF)in effect, provide a theoretical basis for clinical application. Methods Bone marrow adherent cultured rat BMSC SD screening method and collect the super-natant.Intraperitoneal injection of D-galactosamine(D-gal)ALF established rat model;successful model rats were randomly divided into treatment group and control group,the volume of BMSC super-natant ,respectively, and 0.9% sodium chloride injection solution (NS) by intraperitoneal injection 3d, comparing the two groups of animals liver function, pathological changes in liver tissue and 7d survival.Results (1)7d survival rates were : BMSC supernatant 70% of the treatment group , 20% of the control group , there were significant differences (P<0.05). (2)ALT BMSC supernatant treatment group, AST, TBIL significantly reduced, while the control group did not change signifi-cantly impaired liver function, there was a significant difference (P<0.01) between the two.(3)BMSC treated supernatant of liver histological damage significantly improved. Conclusion BMSC super-natant intraperitoneal injection therapy can significantly improve the ALF rat liver function and liver histological damage and improve survival in rats with ALF,ALF treatment to provide a new way.
