中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
22期
10154-10157
,共4页
谢少庭%李绪城%唐小玲%史伟%章斌
謝少庭%李緒城%唐小玲%史偉%章斌
사소정%리서성%당소령%사위%장빈
阿米洛利%蛋白尿%脂多糖类%尿激酶受体
阿米洛利%蛋白尿%脂多糖類%尿激酶受體
아미락리%단백뇨%지다당류%뇨격매수체
Amiloride%Proteinuria%Lipopolysaccharides%Urokinase receptor
目的：观察阿米洛利在脂多糖诱导小鼠短暂蛋白尿模型中对蛋白尿的影响，探讨其影响蛋白尿的机制。方法构建脂多糖（LPS）诱导小鼠短暂蛋白尿模型，实验分为3组：正常对照组（Con）、LPS处理组、LPS与amiloride共同处理组（LPS＋amiloride）。采用考马斯亮蓝法检测各组24 h尿蛋白，利用免疫荧光共聚焦、实时荧光定量PCR等技术，检测足细胞尿激酶受体（uPAR）蛋白和uPAR mRNA的表达情况。结果 LPS组24 h蛋白尿明显高于Con组[（4.12±1.06）mg vs.（1.35±0.68）mg；t＝2.77，P＝0.000]及LPS＋amiloride组[（4.12±1.06）mg vs.（1.99±0.96）mg；t＝2.13，P＝0.001]，均有统计学意义；LPS＋amiloride组24 h蛋白尿与Con组相比无统计学意义（t＝0.64，P＝0.244）；激光共聚焦显微镜观察，LPS组足细胞uPAR表达明显高于Con组与LPS＋amiloride组；LPS组uPAR mRNA表达明显高于Con组（2.12±0.35 vs.1.04±0.14，t＝1.12，P＝0.000）及LPS＋amiloride组（2.12±0.35 vs.1.30±0.22，t＝0.82，P＝0.000），均有统计学意义。结论阿米洛利可能通过抑制受损足细胞uPAR的表达，从而起到降蛋白尿的作用。
目的：觀察阿米洛利在脂多糖誘導小鼠短暫蛋白尿模型中對蛋白尿的影響，探討其影響蛋白尿的機製。方法構建脂多糖（LPS）誘導小鼠短暫蛋白尿模型，實驗分為3組：正常對照組（Con）、LPS處理組、LPS與amiloride共同處理組（LPS＋amiloride）。採用攷馬斯亮藍法檢測各組24 h尿蛋白，利用免疫熒光共聚焦、實時熒光定量PCR等技術，檢測足細胞尿激酶受體（uPAR）蛋白和uPAR mRNA的錶達情況。結果 LPS組24 h蛋白尿明顯高于Con組[（4.12±1.06）mg vs.（1.35±0.68）mg；t＝2.77，P＝0.000]及LPS＋amiloride組[（4.12±1.06）mg vs.（1.99±0.96）mg；t＝2.13，P＝0.001]，均有統計學意義；LPS＋amiloride組24 h蛋白尿與Con組相比無統計學意義（t＝0.64，P＝0.244）；激光共聚焦顯微鏡觀察，LPS組足細胞uPAR錶達明顯高于Con組與LPS＋amiloride組；LPS組uPAR mRNA錶達明顯高于Con組（2.12±0.35 vs.1.04±0.14，t＝1.12，P＝0.000）及LPS＋amiloride組（2.12±0.35 vs.1.30±0.22，t＝0.82，P＝0.000），均有統計學意義。結論阿米洛利可能通過抑製受損足細胞uPAR的錶達，從而起到降蛋白尿的作用。
목적：관찰아미락리재지다당유도소서단잠단백뇨모형중대단백뇨적영향，탐토기영향단백뇨적궤제。방법구건지다당（LPS）유도소서단잠단백뇨모형，실험분위3조：정상대조조（Con）、LPS처리조、LPS여amiloride공동처리조（LPS＋amiloride）。채용고마사량람법검측각조24 h뇨단백，이용면역형광공취초、실시형광정량PCR등기술，검측족세포뇨격매수체（uPAR）단백화uPAR mRNA적표체정황。결과 LPS조24 h단백뇨명현고우Con조[（4.12±1.06）mg vs.（1.35±0.68）mg；t＝2.77，P＝0.000]급LPS＋amiloride조[（4.12±1.06）mg vs.（1.99±0.96）mg；t＝2.13，P＝0.001]，균유통계학의의；LPS＋amiloride조24 h단백뇨여Con조상비무통계학의의（t＝0.64，P＝0.244）；격광공취초현미경관찰，LPS조족세포uPAR표체명현고우Con조여LPS＋amiloride조；LPS조uPAR mRNA표체명현고우Con조（2.12±0.35 vs.1.04±0.14，t＝1.12，P＝0.000）급LPS＋amiloride조（2.12±0.35 vs.1.30±0.22，t＝0.82，P＝0.000），균유통계학의의。결론아미락리가능통과억제수손족세포uPAR적표체，종이기도강단백뇨적작용。
Objective To observe the effect of amiloride on the proteinuria of the LPS mouse model of transient proteinuria. Methods To establish the LPS mouse model of transient proteinuria and divide the experiment into 3 groups, normal control group (Con), LPS induced group (LPS) and amiloride treated group (LPS+amiloride). The concentration of protein and the expression of urokinase receptor (uPAR) and the change of podocytes motility were detected by coomassiebluestaining, immunofluorence method and real-time PCR, etc. Results Comparing with LPS group, the 24 h urine protein of proteinuria in LPS mice treated with amiloride[(4.12±1.06)mg vs. (1.99±0.96) mg; t=2.77, P=0.001] and Con group [(4.12±1.06) mg vs. (1.35±0.68) mg; t=2.13, P=0.000] were significantly reduced. Observed by confocal microscop, compared among the other two groups, a significantly increased induction of uPAR protein expression was observed in LPS induced group. Comparing with Con group, the expression of uPAR mRNA of LPS group was significantly increased (2.12±0.35 vs. 1.04±0.14, t=1.12, P=0.000). In contrast, the expression of uPAR mRNA in LPS mice treated with amiloride was significantly lower than in LPS group (2.12±0.35 vs. 1.30±0.22, t=0.82, P=0.000). Conclusion Amiloride could reduce the proteinuria of the LPS mouse model of transient proteinuria by inhibiting the induction of uPAR expression.
