CAJ | 학술논문

目的：探讨妊娠期高血压胎盘组织中 TGF-β1及 Smad 的表达及意义。方法应用 RT-PCR方法及免疫组化法检测胎盘组织TGF-β1及Smad的表达。结果 RT-PCR结果显示：子痫前期胎盘组织中TGF-β1及Smad mRNA的表达量均增加。重度子痫前期胎盘组织中TGF-β1及Smad mRNA的表达量与正常妊娠组相比有统计学差异（P＜0.01），轻度子痫前期组中的表达与正常妊娠组间有统计学差异（P＜0.05）。免疫组化法显示胎盘组织中TGF-β1及Smad阳性着色部位均位于胞浆，细胞定位于绒毛小叶的蜕膜细胞、合体滋养细胞和细胞滋养细胞。TGF-β1及Smad积分光密度值在妊娠期高血压胎盘中比正常组胎盘中增高明显，具有显著差异，有统计学意义（P＜0.05）。结论胎盘组织中 TGF-β1及 Smad 的表达升高与妊娠期高血压疾病的发病有关。
목적：탐토임신기고혈압태반조직중 TGF-β1급 Smad 적표체급의의。방법응용 RT-PCR방법급면역조화법검측태반조직TGF-β1급Smad적표체。결과 RT-PCR결과현시：자간전기태반조직중TGF-β1급Smad mRNA적표체량균증가。중도자간전기태반조직중TGF-β1급Smad mRNA적표체량여정상임신조상비유통계학차이（P＜0.01），경도자간전기조중적표체여정상임신조간유통계학차이（P＜0.05）。면역조화법현시태반조직중TGF-β1급Smad양성착색부위균위우포장，세포정위우융모소협적세막세포、합체자양세포화세포자양세포。TGF-β1급Smad적분광밀도치재임신기고혈압태반중비정상조태반중증고명현，구유현저차이，유통계학의의（P＜0.05）。결론태반조직중 TGF-β1급 Smad 적표체승고여임신기고혈압질병적발병유관。
Objective To investigate the expression of transforming growth factor-β1 and Smad in placenta of patients with hypertensive disorder complicating pregnancy and their significance. Methods TGF-β1/Smad expressions were detected by immunohistochemical staining in placenta of patients with hypertensive disorder complicating pregnancy, RT-PCR was used to evaluate the expression of TGF-β1/Smad in placental. Results TGF-β1/Smad expressed in decidual cells and syncytiotrophoblast. The expression of TGFβ1/Smad expression levels in the study group were both significantly higher than those in the control group. Conclusion The elevated expression of TGFβ1/Smad may participate in the pathogenesis of hypertensive disorder complicating pregnancy.