CAJ | 학술논문

目的：研究自噬在原发性胆汁性肝硬化（PBC）肝细胞损伤中的作用。方法采用免疫组织化学方法观察PBC患者肝细胞和胆管上皮细胞内微管相关蛋白1轻链3B（LC3B）和自噬相关蛋白beclin1在肝组织的表达，采用透射电镜观察肝细胞和胆管上皮细胞的超微结构改变。结果免疫组化染色显示LC3B和beclin1在PBC患者肝细胞和胆管上皮细胞的细胞质内均有表达，透射电镜下在肝细胞和胆管上皮细胞也有明显的自噬体，同时伴随肝细胞和胆管上皮细胞的损伤。结论自噬可能在PBC患者肝细胞和胆管上皮细胞的损伤中起一定作用。
목적：연구자서재원발성담즙성간경화（PBC）간세포손상중적작용。방법채용면역조직화학방법관찰PBC환자간세포화담관상피세포내미관상관단백1경련3B（LC3B）화자서상관단백beclin1재간조직적표체，채용투사전경관찰간세포화담관상피세포적초미결구개변。결과면역조화염색현시LC3B화beclin1재PBC환자간세포화담관상피세포적세포질내균유표체，투사전경하재간세포화담관상피세포야유명현적자서체，동시반수간세포화담관상피세포적손상。결론자서가능재PBC환자간세포화담관상피세포적손상중기일정작용。
Objective To investigate the role of autophagy in the liver damage of primary biliary cirrhosis (PBC). Methods The expression of microtubule-associated protein 1 light chain-3B(LC3B) and beclin 1 was detected by immunohistochemistry, the ultrastructural changes of liver tissues was observed by transmission electron microscopy. Results The immunohistochemistry showed that LC3B and beclin 1 were expressed in the hepatocytes and biliary epithelial cells of PBC patients. The autophagic bodies were found in both hepatocytes and biliary epithelial cells of PBC patients, and the damage of hepatocytes and biliary epithelial cells were also found simultaneously. Conclusion Autophagy may be involved in the damage of hepatocytes and biliary epithelial cells of PBC.