CAJ | 학술논문

目的：探讨自噬标志物--微管相关蛋白轻链3B（MAPLC3B）在人肺鳞癌组织中的表达以及与窖蛋白-1（Cav-1）的关系及它们的临床意义。方法利用量子点免疫荧光组织化学技术检测LC3B和Cav-1蛋白在肺鳞癌组织芯片包括100例肺鳞癌组织和20例非癌性肺组织上的表达；同时在20例肺鳞癌和20例非癌性肺组织中利用定量实时PCR法检测LC3B和Cav-1 mRNA的变化。结果与非癌变肺组织相比，LC3B和Cav-1蛋白和mRNA在肺鳞癌组织的表达均显著降低（P＜0.01）。LC3B 蛋白阳性表达与男性肺鳞癌患者显著相关，与淋巴结转移相关，但差异无显著性（P＞0.05）。肿瘤细胞Cav-1蛋白阳性表达在肺鳞癌TNM分期（P＝0.009）和淋巴结有无转移（P＝0.027）之间的差异均有显著性，而与其他临床病理参数均无关（P＞0.05）。基质Cav-1阳性率仅为10.12%（8/79），与肺鳞癌临床病理参数均无关（P＞0.05）。LC3B和Cav-1蛋白在肺鳞癌肿瘤细胞的表达呈显著正相关（P＝0.001，r＝0.473）。结论自噬基因 LC3B 和 Cav-1的缺失表达可能与肺鳞癌的发生有关，但二者共同阳性表达又可协同促进肿瘤的恶性进展。
목적：탐토자서표지물--미관상관단백경련3B（MAPLC3B）재인폐린암조직중적표체이급여교단백-1（Cav-1）적관계급타문적림상의의。방법이용양자점면역형광조직화학기술검측LC3B화Cav-1단백재폐린암조직심편포괄100례폐린암조직화20례비암성폐조직상적표체；동시재20례폐린암화20례비암성폐조직중이용정량실시PCR법검측LC3B화Cav-1 mRNA적변화。결과여비암변폐조직상비，LC3B화Cav-1단백화mRNA재폐린암조직적표체균현저강저（P＜0.01）。LC3B 단백양성표체여남성폐린암환자현저상관，여림파결전이상관，단차이무현저성（P＞0.05）。종류세포Cav-1단백양성표체재폐린암TNM분기（P＝0.009）화림파결유무전이（P＝0.027）지간적차이균유현저성，이여기타림상병리삼수균무관（P＞0.05）。기질Cav-1양성솔부위10.12%（8/79），여폐린암림상병리삼수균무관（P＞0.05）。LC3B화Cav-1단백재폐린암종류세포적표체정현저정상관（P＝0.001，r＝0.473）。결론자서기인 LC3B 화 Cav-1적결실표체가능여폐린암적발생유관，단이자공동양성표체우가협동촉진종류적악성진전。
Objective To detect autophagy marker-microtubule associated protein light chain 3 (LC3B) expression and its correlation with caveolin-1 (Cav-1) in human lung squamous cell carcinoma(SCC) tissues and to investigate their clinicopathologic significance. Methods The quantum dots immunofluorescent histochemistry was used to detect the expression of LC3B and Cav-1 proteins in 100 cases of lung SCC and 20 cases of noncancerous lung tissues, and mRNA levels of LC3B and Cav-1 were detected by quantitative real-time PCR methods in the 20 cases of lung SCC and 20 cases of noncancerous lung tissues. Results Compared to noncancerous lung tissues, proteins and mRNA levels of LC3B and Cav-1 in the lung SCC tissues were significantly decreased(P<0.05). Positive expression of LC3B protein was positively correlated with male lung SCC patients, related with lymph node metastasis, but no significant differences was observed(P>0.05). There were significant differences of Cav-1 protein expression in the tumor cells between clinical TNM stage(P=0.009) and lymph node metastasis(P=0.027), but Cav-1 protein expression was not correlated with the other clinicopathologic parameters(P>0.05). Positive rates of stromal Cav-1 was 10.12%(8/79), not correlated with the clinicopathologic parameters of lung SCC (P>0.05). There was positive correlation between LC3B and Cav-1 in the tumor cells of lung SCC tissues(P<0.05, rs=0.473). Conclusion Absent expression of autophagy gene LC3B and Cav-1 may be correlated with the development of lung squamous cell carcinoma, however, positive co-expression of LC3B and Cav-1 may synergistically promote malignant progression of tumors.