中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
22期
10001-10005
,共5页
刘小刚%乔帆%陆方林%徐志云
劉小剛%喬帆%陸方林%徐誌雲
류소강%교범%륙방림%서지운
心脏缺损, 先天性%高血压, 肺性%骨形态发生蛋白受体,Ⅱ型%miRNA%血管重构
心髒缺損, 先天性%高血壓, 肺性%骨形態髮生蛋白受體,Ⅱ型%miRNA%血管重構
심장결손, 선천성%고혈압, 폐성%골형태발생단백수체,Ⅱ형%miRNA%혈관중구
Heart defects,congenital%Hypertension,pulmonary%Bone morphogenetic protein receptors,type II%microRNA%Vascular remodeling
目的:探讨 miR-19a 与骨形成蛋白2型受体(BMPR2)在先天性心脏病相关性肺动脉高压(CHD-PAH)患者肺组织中表达水平变化与肺动脉血管重构之间的可能关系。方法以18例CHD-PAH患者肺组织为试验组,18例自发性气胸患者术中切除的正常肺组织为对照组。通过免疫组化染色观察肺动脉中BMPR2表达水平;Western-blotting检测试验组与对照组中BMPR2的相对含量;qRT-PCR技术对重度肺动脉高压患者及正常人肺组织中BMPR2 mRNA、miR-19a表达量进行检测。结果免疫组化结果提示试验组 BMPR2在肺动脉中表达较正常对照组显著减少(F=9.65,P<0.01),且与肺动脉病理分级呈负相关(r=-0.7379,P<0.001),Western-blotting 检测结果提示 BMPR2蛋白表达量在试验组肺组织中较正常对照组表达显著下降(0.138±0.002 vs.0.069±0.001;P<0.0001)。qRT-PCR结果提示试验组较正常对照组BMPR2 mRNA表达显著下降(6.370±0.212 vs.2.358±0.086;P<0.0001),而miR-19a表达显著升高(2.555±0.098 vs.54.27±2.574;P<0.0001)。结论 BMPR2在CHD-PAH患者肺动脉中表达减少,其在 CHD-PAH 肺动脉重构中可能具有一定作用,miR-19a可能参与 CHD-PAH肺组织中 BMPR2的调控表达。
目的:探討 miR-19a 與骨形成蛋白2型受體(BMPR2)在先天性心髒病相關性肺動脈高壓(CHD-PAH)患者肺組織中錶達水平變化與肺動脈血管重構之間的可能關繫。方法以18例CHD-PAH患者肺組織為試驗組,18例自髮性氣胸患者術中切除的正常肺組織為對照組。通過免疫組化染色觀察肺動脈中BMPR2錶達水平;Western-blotting檢測試驗組與對照組中BMPR2的相對含量;qRT-PCR技術對重度肺動脈高壓患者及正常人肺組織中BMPR2 mRNA、miR-19a錶達量進行檢測。結果免疫組化結果提示試驗組 BMPR2在肺動脈中錶達較正常對照組顯著減少(F=9.65,P<0.01),且與肺動脈病理分級呈負相關(r=-0.7379,P<0.001),Western-blotting 檢測結果提示 BMPR2蛋白錶達量在試驗組肺組織中較正常對照組錶達顯著下降(0.138±0.002 vs.0.069±0.001;P<0.0001)。qRT-PCR結果提示試驗組較正常對照組BMPR2 mRNA錶達顯著下降(6.370±0.212 vs.2.358±0.086;P<0.0001),而miR-19a錶達顯著升高(2.555±0.098 vs.54.27±2.574;P<0.0001)。結論 BMPR2在CHD-PAH患者肺動脈中錶達減少,其在 CHD-PAH 肺動脈重構中可能具有一定作用,miR-19a可能參與 CHD-PAH肺組織中 BMPR2的調控錶達。
목적:탐토 miR-19a 여골형성단백2형수체(BMPR2)재선천성심장병상관성폐동맥고압(CHD-PAH)환자폐조직중표체수평변화여폐동맥혈관중구지간적가능관계。방법이18례CHD-PAH환자폐조직위시험조,18례자발성기흉환자술중절제적정상폐조직위대조조。통과면역조화염색관찰폐동맥중BMPR2표체수평;Western-blotting검측시험조여대조조중BMPR2적상대함량;qRT-PCR기술대중도폐동맥고압환자급정상인폐조직중BMPR2 mRNA、miR-19a표체량진행검측。결과면역조화결과제시시험조 BMPR2재폐동맥중표체교정상대조조현저감소(F=9.65,P<0.01),차여폐동맥병리분급정부상관(r=-0.7379,P<0.001),Western-blotting 검측결과제시 BMPR2단백표체량재시험조폐조직중교정상대조조표체현저하강(0.138±0.002 vs.0.069±0.001;P<0.0001)。qRT-PCR결과제시시험조교정상대조조BMPR2 mRNA표체현저하강(6.370±0.212 vs.2.358±0.086;P<0.0001),이miR-19a표체현저승고(2.555±0.098 vs.54.27±2.574;P<0.0001)。결론 BMPR2재CHD-PAH환자폐동맥중표체감소,기재 CHD-PAH 폐동맥중구중가능구유일정작용,miR-19a가능삼여 CHD-PAH폐조직중 BMPR2적조공표체。
Objective To study the association between bone morphogenetic protein receptor 2 (BMPR2) expression level and miR-19a expression level in the lung tissue of pulmonary arterial hypertension associated with congenital heart disease(CHD-PAH), then make out the relationships between their changes and pulmonary vascular remodeling. Methods The lung tissue of 18 patients with CHD-PAH for the experimental group, and normal lung tissue of 18 patients with spontaneous pneumothorax undergoing resection as control group. Detected the BMPR2 expression around pulmonary vascular with immunohistochemical staining and western-blotting. and the expression level of BMPR2 mRNA and miR-19a with qRT-PCR. Results Compared to the control group, BMPR2 protein and mRNA were decreased in the lung tissue of experimental group. But the expression of miR-19a was increased in the lung tissue of experimental group (2.555±0.098 vs. 54.27±2.574; P<0.0001). Conclusion BMPR2 expression is decreased in the lung tissue of CHD-PAH, it may take part in the remodeling procedure of pulmonary artery. miR-19a might modulate BMPR2 expression in vascular remodeling procedure of CHD-PAH.