中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
19期
2909-2911
,共3页
何氏加味定喘汤%慢性支气管炎%大鼠
何氏加味定喘湯%慢性支氣管炎%大鼠
하씨가미정천탕%만성지기관염%대서
Ho flavored Dingchuan Decoction%Chronic bronchitis%Rat
目的 观察何氏加味定喘汤对慢性支气管炎大鼠氧化/抗氧化相关指标的影响.方法 SD大鼠50只,采用改良烟熏法复制慢性支气管炎模型,从造模第20天起,空白对照组及模型对照组用0.9％氯化钠注射液1 ml/100 g,给药组按1 ml/100 g体积灌胃,阳性对照组用止嗽合剂(5g·kg-1·d-1)灌胃,何氏加味定喘汤高剂量组(20g·kg-1· d-1)、何氏加味定喘汤中剂量组(10 g·kg-1·d-1)、何氏加味定喘汤低剂量组(5 g·kg-1·d-1)分别灌胃,各组灌胃均为每天1次.各组均用药20 d.观察治疗后大鼠血清、肺组织匀浆、肺泡灌流液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及丙二醛(MDA)含量.结果 慢性支气管炎模型组大鼠血清、肺组织匀浆及肺泡灌洗液中SOD[(188.11±9.37) U/ml、(276.11±30.28) U/ml、(8.80±1.49)U/rml]、CAT(9.40±2.04) U/ml、(26.57 ±4.26) U/ml、(1.58±0.26)U/ml)]等清除自由基的酶类活性,较空白对照组SOD、CAT显著降低(P＜0.01)；血清、肺组织匀浆及肺泡灌洗液中的脂质过氧化物MDA[(5.64 ±0.52) μmol/L、(6.32±2.10)μmol/L、(28.08±2.43)μmol/L]]的含量较空白对照组增加(P＜0.05或P＜0.01),何氏加味定喘汤可有效地逆转这些改变(P＜0.05或P＜0.01),并呈明显的剂量依赖性,何氏加味定喘汤中、高剂量组大鼠血清中SOD[(253.05±7.81) U/ml、(256.92±5.37) U/ml]、CAT(14.86±2.49) U/ml、(18.22±2.65) U/ml)]活性比空白对照组还略高,何氏加味定喘汤中、高剂量组大鼠及肺组织匀浆中SOD[(470.91±11.46) U/mgprot、(482.91±14.32) U/mgprot]、CAT(46.87±3.75) U/mgprot、(55.49±3.33) U/mgprot)]活性亦比空白对照组还高.何氏加味定喘汤高剂量组大鼠血清及肺组织匀浆中MDA含量[(3.57±1.04)μmol/L]接近正常组水平.何氏加味定喘汤高剂量组大鼠肺泡灌流液中SOD(16.15±0.86) U/ml、CAT(2.88±0.25) U/ml活性和MDA(15.18±2.25)μmol/L含量均接近正常组水平.结论 何氏加味定喘汤对慢性支气管炎具有明显的减轻氧化应激作用.
目的 觀察何氏加味定喘湯對慢性支氣管炎大鼠氧化/抗氧化相關指標的影響.方法 SD大鼠50隻,採用改良煙熏法複製慢性支氣管炎模型,從造模第20天起,空白對照組及模型對照組用0.9％氯化鈉註射液1 ml/100 g,給藥組按1 ml/100 g體積灌胃,暘性對照組用止嗽閤劑(5g·kg-1·d-1)灌胃,何氏加味定喘湯高劑量組(20g·kg-1· d-1)、何氏加味定喘湯中劑量組(10 g·kg-1·d-1)、何氏加味定喘湯低劑量組(5 g·kg-1·d-1)分彆灌胃,各組灌胃均為每天1次.各組均用藥20 d.觀察治療後大鼠血清、肺組織勻漿、肺泡灌流液中超氧化物歧化酶(SOD)、過氧化氫酶(CAT)及丙二醛(MDA)含量.結果 慢性支氣管炎模型組大鼠血清、肺組織勻漿及肺泡灌洗液中SOD[(188.11±9.37) U/ml、(276.11±30.28) U/ml、(8.80±1.49)U/rml]、CAT(9.40±2.04) U/ml、(26.57 ±4.26) U/ml、(1.58±0.26)U/ml)]等清除自由基的酶類活性,較空白對照組SOD、CAT顯著降低(P＜0.01)；血清、肺組織勻漿及肺泡灌洗液中的脂質過氧化物MDA[(5.64 ±0.52) μmol/L、(6.32±2.10)μmol/L、(28.08±2.43)μmol/L]]的含量較空白對照組增加(P＜0.05或P＜0.01),何氏加味定喘湯可有效地逆轉這些改變(P＜0.05或P＜0.01),併呈明顯的劑量依賴性,何氏加味定喘湯中、高劑量組大鼠血清中SOD[(253.05±7.81) U/ml、(256.92±5.37) U/ml]、CAT(14.86±2.49) U/ml、(18.22±2.65) U/ml)]活性比空白對照組還略高,何氏加味定喘湯中、高劑量組大鼠及肺組織勻漿中SOD[(470.91±11.46) U/mgprot、(482.91±14.32) U/mgprot]、CAT(46.87±3.75) U/mgprot、(55.49±3.33) U/mgprot)]活性亦比空白對照組還高.何氏加味定喘湯高劑量組大鼠血清及肺組織勻漿中MDA含量[(3.57±1.04)μmol/L]接近正常組水平.何氏加味定喘湯高劑量組大鼠肺泡灌流液中SOD(16.15±0.86) U/ml、CAT(2.88±0.25) U/ml活性和MDA(15.18±2.25)μmol/L含量均接近正常組水平.結論 何氏加味定喘湯對慢性支氣管炎具有明顯的減輕氧化應激作用.
목적 관찰하씨가미정천탕대만성지기관염대서양화/항양화상관지표적영향.방법 SD대서50지,채용개량연훈법복제만성지기관염모형,종조모제20천기,공백대조조급모형대조조용0.9％록화납주사액1 ml/100 g,급약조안1 ml/100 g체적관위,양성대조조용지수합제(5g·kg-1·d-1)관위,하씨가미정천탕고제량조(20g·kg-1· d-1)、하씨가미정천탕중제량조(10 g·kg-1·d-1)、하씨가미정천탕저제량조(5 g·kg-1·d-1)분별관위,각조관위균위매천1차.각조균용약20 d.관찰치료후대서혈청、폐조직균장、폐포관류액중초양화물기화매(SOD)、과양화경매(CAT)급병이철(MDA)함량.결과 만성지기관염모형조대서혈청、폐조직균장급폐포관세액중SOD[(188.11±9.37) U/ml、(276.11±30.28) U/ml、(8.80±1.49)U/rml]、CAT(9.40±2.04) U/ml、(26.57 ±4.26) U/ml、(1.58±0.26)U/ml)]등청제자유기적매류활성,교공백대조조SOD、CAT현저강저(P＜0.01)；혈청、폐조직균장급폐포관세액중적지질과양화물MDA[(5.64 ±0.52) μmol/L、(6.32±2.10)μmol/L、(28.08±2.43)μmol/L]]적함량교공백대조조증가(P＜0.05혹P＜0.01),하씨가미정천탕가유효지역전저사개변(P＜0.05혹P＜0.01),병정명현적제량의뢰성,하씨가미정천탕중、고제량조대서혈청중SOD[(253.05±7.81) U/ml、(256.92±5.37) U/ml]、CAT(14.86±2.49) U/ml、(18.22±2.65) U/ml)]활성비공백대조조환략고,하씨가미정천탕중、고제량조대서급폐조직균장중SOD[(470.91±11.46) U/mgprot、(482.91±14.32) U/mgprot]、CAT(46.87±3.75) U/mgprot、(55.49±3.33) U/mgprot)]활성역비공백대조조환고.하씨가미정천탕고제량조대서혈청급폐조직균장중MDA함량[(3.57±1.04)μmol/L]접근정상조수평.하씨가미정천탕고제량조대서폐포관류액중SOD(16.15±0.86) U/ml、CAT(2.88±0.25) U/ml활성화MDA(15.18±2.25)μmol/L함량균접근정상조수평.결론 하씨가미정천탕대만성지기관염구유명현적감경양화응격작용.
Objective To study chronic bronchitis rats oxidant/antioxidant related indicators Ho flavored Dingchuan Decoction.Methods 50 SD rats were adopted improved smoked method to make chronic bronchitis model.From the 20th day of the modeling,blank control group and model control group gavage were treated with saline.The positive control group were treated with Cough Mixture.The experimental groupwere dealed with Ho flavored Dingchuan Decoction high,medium and low-dose.20 day later,the cotent of SOD,CAT,MDA in rat serum,lung tissue and balf were observed.Results Compare to blank control group,the cotent of SOD[(188.11 ± 9.37)U/ml、(276.11 ±30.28) U/ml,(8.80 ± 1.49)U/ml],CAT[(9.40 ±2.04)U/ml,(26.57±4.26)U/ml,(1.58±0.26) U/ml] in rat serum,lung tissue of the experimental group significantly reduced (P ＜0.01) ; MDA [(5.64 ± 0.52) μmol/L,(6.32 ± 2.10) μmol/L,(28.08 ± 2.43) μ mol/L) increased (P ＜0.05 or P ＜ 0.01).Ho flavored Dingchuan Decoction can effectively reverse these changes,and showed a significant dose-dependent.The Ho flavored Dingchuantang,in middle and high-dose serum SOD [(253.05 ± 7.81) U/ml,(256.92 ± 5.37)U/ml],CAT [(14.86 ± 2.49)U/ml,(18.22 ± 2.65)U/ml] higher than those of blank control group.And the Ho flavored Dingchuantang,in middle and high-dose lung tissue SOD [(470.91 ± 11.46)U/mgprot,(482.91 ± 14.32)U/mgprot),CAT [(46.87 ± 3.75)U/mgprot,(55.49 ±3.33)U/mgprot] higher than those of blank control group.Content close to the normal level.Conclusion The Ho flavored Dingchuan Decoction has significantly reduce the oxidative stress in chronic bronchitis.
