CAJ | 학술논문

评价交叉引物恒温扩增方法对食品中凝固酶阳性的金黄色葡萄球菌的检测效果。应用针对凝固酶阳性的金黄色葡萄球菌建立等温扩增方法对5类15种食品进行凝固酶阳性金黄色葡萄球菌检测，根据ISO 5725-2：1994标准要求，以现行国家标准为基准方法，对交叉引物-待确认方法进行灵敏度、特异性、假阳性率、假阴性率及相对准确度等五方面验证。建立的交叉引物等温扩增方法灵敏度100%、特异性98.1%、假阴性率0、假阳性率1.9%、相对准确度98.3%。
평개교차인물항온확증방법대식품중응고매양성적금황색포도구균적검측효과。응용침대응고매양성적금황색포도구균건립등온확증방법대5류15충식품진행응고매양성금황색포도구균검측，근거ISO 5725-2：1994표준요구，이현행국가표준위기준방법，대교차인물-대학인방법진행령민도、특이성、가양성솔、가음성솔급상대준학도등오방면험증。건립적교차인물등온확증방법령민도100%、특이성98.1%、가음성솔0、가양성솔1.9%、상대준학도98.3%。
Evaluation of the effect on cross-primer isothermal amplification method for detection of coagulase positive Staphylococcus aureus on food. To detect coagulase positive Staphylococcus aureus on the five types and fifteen foods by using the cross-primer isothermal amplification method. According to the ISO standard , confirm the cross-primer isothermal amplification method from the sensitivity, specificity, false positive rate, false negative rate and relative accuracy , the current national standard was the reference method. The method established in this article was highly specific and sensitive compare with the reference method. The sensitivity 100%, specificity98.1%, false positive rate1.9%, false negative rate0 and relative accuracy 98.3%.