食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
24期
197-200
,共4页
桑葚%花色苷%矢车菊素-3-葡萄糖苷%高效液相色谱-电喷雾质谱
桑葚%花色苷%矢車菊素-3-葡萄糖苷%高效液相色譜-電噴霧質譜
상심%화색감%시차국소-3-포도당감%고효액상색보-전분무질보
mulberry%anthocyanins%cyanidin-3-glucoside%HPLC-ESI-MS
采用高效液相色谱-电喷雾质谱（HPLC-ESI-MS）法测定桑葚中花色苷含量及种类。Zorbax SB-C18色谱柱（250 mm ×4.6 mm，5μm），流动相为乙腈-10%甲酸水溶液，流速1.0 mL/min，检测波长520 nm。以矢车菊素-3-葡萄糖苷（Cy-3-G）为标准，外标法定量；并通过离子碎片对花色苷组成进行定性分析。结果发现，每100克桑葚鲜果含花色苷（711.7±49.5） mg；分别为矢车菊素-3-（2G-葡糖芸香糖苷）、飞燕草素-3-芸香糖苷-5-葡萄糖苷、矢车菊素-3，5-二葡萄糖苷、矢车菊素-3-葡萄糖苷、矢车菊素-3-芸香糖苷、天竺葵素-3-葡萄糖苷、天竺葵素-3-芸香糖苷和飞燕草素-3-芸香糖苷等8种花色苷。
採用高效液相色譜-電噴霧質譜（HPLC-ESI-MS）法測定桑葚中花色苷含量及種類。Zorbax SB-C18色譜柱（250 mm ×4.6 mm，5μm），流動相為乙腈-10%甲痠水溶液，流速1.0 mL/min，檢測波長520 nm。以矢車菊素-3-葡萄糖苷（Cy-3-G）為標準，外標法定量；併通過離子碎片對花色苷組成進行定性分析。結果髮現，每100剋桑葚鮮果含花色苷（711.7±49.5） mg；分彆為矢車菊素-3-（2G-葡糖蕓香糖苷）、飛燕草素-3-蕓香糖苷-5-葡萄糖苷、矢車菊素-3，5-二葡萄糖苷、矢車菊素-3-葡萄糖苷、矢車菊素-3-蕓香糖苷、天竺葵素-3-葡萄糖苷、天竺葵素-3-蕓香糖苷和飛燕草素-3-蕓香糖苷等8種花色苷。
채용고효액상색보-전분무질보（HPLC-ESI-MS）법측정상심중화색감함량급충류。Zorbax SB-C18색보주（250 mm ×4.6 mm，5μm），류동상위을정-10%갑산수용액，류속1.0 mL/min，검측파장520 nm。이시차국소-3-포도당감（Cy-3-G）위표준，외표법정량；병통과리자쇄편대화색감조성진행정성분석。결과발현，매100극상심선과함화색감（711.7±49.5） mg；분별위시차국소-3-（2G-포당예향당감）、비연초소-3-예향당감-5-포도당감、시차국소-3，5-이포도당감、시차국소-3-포도당감、시차국소-3-예향당감、천축규소-3-포도당감、천축규소-3-예향당감화비연초소-3-예향당감등8충화색감。
Investigation of the content and variety of anthocyanins in mulberry by high-performance liquid chromatography with electrospray ionization-mass spectrometry (HPLC-ESI-MS). The analysis was carried on Zorbax SB-C18 column (250 mm × 4.6 mm, 5μm). The mobile phase was acetonitrile and 10%formic acid-water at the flow rate of 1.0 mL/min, detection wavelength was 520 nm. Mulberry anthocyanins were quantitated by cyanidin-3-glucoside according to external reference method and identified on the basis of ion fragments. The results showed that about (711.7 ±49.5) mg anthocyanins in 100 g fresh mulberry; eight kinds of anthocyanins were found in mulberry, such as cyanidin-3-(2G-glucosylrutinoside), delphinidin-3-rutinoside-5-glucoside, cyanidin-3,5-diglucoside, cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside, and delphinidin-3-rutinoside.
