CAJ | 학술논문

建立一种PCR方法可以检测食品包装材料中沙门氏菌。参考文献中沙门氏菌的invA基因序列设计引物，通过PCR对沙门氏菌的目的基因进行扩增，并与其它常见致病菌同时进行多重PCR检测。能特异地扩增出284 bp的目的条带，检出限可达到0.8551 pg。该方法具有良好的检测特异性，可实现沙门氏菌的快速检测。
건립일충PCR방법가이검측식품포장재료중사문씨균。삼고문헌중사문씨균적invA기인서렬설계인물，통과PCR대사문씨균적목적기인진행확증，병여기타상견치병균동시진행다중PCR검측。능특이지확증출284 bp적목적조대，검출한가체도0.8551 pg。해방법구유량호적검측특이성，가실현사문씨균적쾌속검측。
The purpose of this paper is to establish a PCR method in which Salmonella can be detected in food packaging materials. The primers were designed according to invA gene as references , and we amplified the target gene by single and multiple PCR. Since the detection limit can reach 0.855 1 pg , the detected method is very specific, and can achieve the rapid detection of Salmonella in food packaging materials.