食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
24期
181-185
,共5页
内生真菌%八角%抗氧化%球毛壳菌
內生真菌%八角%抗氧化%毬毛殼菌
내생진균%팔각%항양화%구모각균
endophytic fungi%Illicium verum%antioxidant activity%Chaetomium globosum
采用组织贴片培养分离法，分别从八角果、叶、枝等健康组织中分离纯化获得24、32和23株内生真菌。分别采用总抗氧化性能测定、DPPH自由基清除实验对79株内生真菌抗氧化活性进行了筛选。结果表明，从八角果实和枝条中分离获得的内生真菌BGEF11和BJEF13发酵液具有较强的抗氧化活性，其总抗氧化性能分别达147.11±11.87（μg Vc/mL）和148.16±13.11（μg VC/mL），对DPPH自由基清除率分别为84.84%和93.58%。经形态学鉴定， BGEF11和BJEF13均为球毛壳菌。
採用組織貼片培養分離法，分彆從八角果、葉、枝等健康組織中分離純化穫得24、32和23株內生真菌。分彆採用總抗氧化性能測定、DPPH自由基清除實驗對79株內生真菌抗氧化活性進行瞭篩選。結果錶明，從八角果實和枝條中分離穫得的內生真菌BGEF11和BJEF13髮酵液具有較彊的抗氧化活性，其總抗氧化性能分彆達147.11±11.87（μg Vc/mL）和148.16±13.11（μg VC/mL），對DPPH自由基清除率分彆為84.84%和93.58%。經形態學鑒定， BGEF11和BJEF13均為毬毛殼菌。
채용조직첩편배양분리법，분별종팔각과、협、지등건강조직중분리순화획득24、32화23주내생진균。분별채용총항양화성능측정、DPPH자유기청제실험대79주내생진균항양화활성진행료사선。결과표명，종팔각과실화지조중분리획득적내생진균BGEF11화BJEF13발효액구유교강적항양화활성，기총항양화성능분별체147.11±11.87（μg Vc/mL）화148.16±13.11（μg VC/mL），대DPPH자유기청제솔분별위84.84%화93.58%。경형태학감정， BGEF11화BJEF13균위구모각균。
Used the cultural method of plant tissue paster on solid medium, 24, 32 and 23 strains of endophytic fungi were separated from fine tissue of Illicium verum fruits, leaves and branches, respectively. Antioxidant activities of broth of the 79 strains, such as total antioxidant activity and DPPH radical scavenging activity, were studied by vitro methods. The results showed that BGEF11 and BJEF13 , which isolated from fruits and branches respectively, have strong antioxidant activities. The total antioxidant properties of the two strains broth which expressed asμg Vc/mL were up to 147.11 ± 11.87 and 148.16 ± 13.11, respectively. And the scavenging rates on DPPH radical of the two strains were 84.84 % and 93.58 %, respectively. The two strains were identified as Chaetomium globosum by morphological method.
