中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
14期
29-31
,共3页
过敏性紫癜性肾炎%金属蛋白酶组织抑制物-1%ELISA双抗体夹心法%肾损害
過敏性紫癜性腎炎%金屬蛋白酶組織抑製物-1%ELISA雙抗體夾心法%腎損害
과민성자전성신염%금속단백매조직억제물-1%ELISA쌍항체협심법%신손해
Henoch-Schonlein purpura nephritis%Tissue inhibitor of metalloproteinase-1%Double antibody sandwich ELISA method%Renal damage
目的：观察不同分期过敏性紫癜性肾炎（Henoch-Schonlein purpura nephritis，HSPN）患者和健康人血清中金属蛋白酶组织抑制物-1（tissue inhibitors of metalloproteinase-1，TIMP-1）水平的变化，初步探讨TIMP-1在HSPN不同阶段中的作用及其与肾损害程度的关系，通过相关药物阻断TIMP-1的表达或抑制其活性，可能为预防、治疗肾脏纤维化提供一个新方法，以期达到理想治疗效果。方法：将过敏性紫癜性肾炎患者42例（试验组）按病理类型分组，并设立健康对照组。抽取两组的外周静脉血5 mL，分离血清，应用ELISA双抗体夹心法分别对其进行检测，观察各组TIMP-1水平的表达情况。结果：试验组Ⅱ~Ⅵ级组血清中TIMP-1水平较健康对照组显著增高（P<0.05），Ⅲ~Ⅵ级组血清中TIMP-1水平较Ⅱ级组显著增高（P<0.01），Ⅰ级组血清中TIMP-1水平与健康对照组比较差异无统计学意义（P>0.05）。结论：通过检测TIMP-1水平，再结合其他化验指标和临床表现，有助于对该疾病的治疗效果或者预后给予判断，对减少肾功能衰竭发生率具有指导意义。
目的：觀察不同分期過敏性紫癜性腎炎（Henoch-Schonlein purpura nephritis，HSPN）患者和健康人血清中金屬蛋白酶組織抑製物-1（tissue inhibitors of metalloproteinase-1，TIMP-1）水平的變化，初步探討TIMP-1在HSPN不同階段中的作用及其與腎損害程度的關繫，通過相關藥物阻斷TIMP-1的錶達或抑製其活性，可能為預防、治療腎髒纖維化提供一箇新方法，以期達到理想治療效果。方法：將過敏性紫癜性腎炎患者42例（試驗組）按病理類型分組，併設立健康對照組。抽取兩組的外週靜脈血5 mL，分離血清，應用ELISA雙抗體夾心法分彆對其進行檢測，觀察各組TIMP-1水平的錶達情況。結果：試驗組Ⅱ~Ⅵ級組血清中TIMP-1水平較健康對照組顯著增高（P<0.05），Ⅲ~Ⅵ級組血清中TIMP-1水平較Ⅱ級組顯著增高（P<0.01），Ⅰ級組血清中TIMP-1水平與健康對照組比較差異無統計學意義（P>0.05）。結論：通過檢測TIMP-1水平，再結閤其他化驗指標和臨床錶現，有助于對該疾病的治療效果或者預後給予判斷，對減少腎功能衰竭髮生率具有指導意義。
목적：관찰불동분기과민성자전성신염（Henoch-Schonlein purpura nephritis，HSPN）환자화건강인혈청중금속단백매조직억제물-1（tissue inhibitors of metalloproteinase-1，TIMP-1）수평적변화，초보탐토TIMP-1재HSPN불동계단중적작용급기여신손해정도적관계，통과상관약물조단TIMP-1적표체혹억제기활성，가능위예방、치료신장섬유화제공일개신방법，이기체도이상치료효과。방법：장과민성자전성신염환자42례（시험조）안병리류형분조，병설립건강대조조。추취량조적외주정맥혈5 mL，분리혈청，응용ELISA쌍항체협심법분별대기진행검측，관찰각조TIMP-1수평적표체정황。결과：시험조Ⅱ~Ⅵ급조혈청중TIMP-1수평교건강대조조현저증고（P<0.05），Ⅲ~Ⅵ급조혈청중TIMP-1수평교Ⅱ급조현저증고（P<0.01），Ⅰ급조혈청중TIMP-1수평여건강대조조비교차이무통계학의의（P>0.05）。결론：통과검측TIMP-1수평，재결합기타화험지표화림상표현，유조우대해질병적치료효과혹자예후급여판단，대감소신공능쇠갈발생솔구유지도의의。
Objective:To study the changes of serum tissue inhibitors of metalloproteinase-1(TIMP-1)in different stages of patients with Henoch-Schonlein purpura nephritis(HSPN),to discuss the role of TIMP-1 in HSPN and its relationship with renal damage. By blocking the expression of TIMP-1 or inhibit its activity,to provide a new method for the treatment of renal fibrosis. Method:42 patients with HSPN(experimental group)were divided into six groups according to the pathological types,and normal control group was set up. 5 mL peripheral vein blood from all of the research object were extracted and separated its serum. Double antibody sandwich ELISA method was used to detect the expression of IMP-1 in each group. Result:The levels of serum TIMP-1 in II-VI grade HSPN were significantly higher than those in the normal control group(P<0.05),and its levels inⅢ-Ⅳgrade HSPN were significantly higher than those in the Ⅱ grade(P<0.01). But compared with the normal control group,the levels of TIMP-1 in I grade HSPN had no statistical significance(P>0.05). Conclusion:By detecting TIMP-1 level,in combination with other test indicators and clinical symptoms,can help the treatment of the disease or to judge prognosis,it has the instruction significance to reduce the incidence of renal failure.
