实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2014年
2期
150-152
,共3页
EB病毒%DNA载量%鼻咽癌%临床分期%实时荧光定量PCR
EB病毒%DNA載量%鼻嚥癌%臨床分期%實時熒光定量PCR
EB병독%DNA재량%비인암%림상분기%실시형광정량PCR
Epstein-Barr virus%DNA load%Nasopharyngeal carcinoma%Clinical stage%Real-time quantitative PCR
目的:检测鼻咽癌患者外周血淋巴细胞中EB病毒DNA载量,并分析其与鼻咽癌临床分期的关系。方法选取2012年1月至2013年1月我院肿瘤科收治住院的87例鼻咽癌患者,采用实时荧光定量PCR法检测外周血淋巴细胞EB病毒DNA含量;统计学分析其与患者临床分期的关系。结果鼻咽癌患者外周血淋巴细胞中EB病毒DNA检出率为82.76%(72/87),EB病毒DNA拷贝数的中位数为7.58×106拷贝数/ml,Ⅰ、Ⅱ、Ⅲ和Ⅳ期患者每毫升DNA拷贝数分别为(4.25±1.14)×103、(4.56±0.68)×104、(7.88±1.65)×105和(8.09±1.65)×106;EB病毒DNA载量随着疾病临床分期的增加呈现逐步升高的趋势,且差异显著(P<0.05)。结论鼻咽癌患者外周血淋巴细胞中EB病毒DNA载量与患者临床分期相关,提示EB病毒DNA载量与鼻咽癌病情进展关系密切,在临床上可作为鼻咽癌患者病情监测及判断疗效的一种有效手段。
目的:檢測鼻嚥癌患者外週血淋巴細胞中EB病毒DNA載量,併分析其與鼻嚥癌臨床分期的關繫。方法選取2012年1月至2013年1月我院腫瘤科收治住院的87例鼻嚥癌患者,採用實時熒光定量PCR法檢測外週血淋巴細胞EB病毒DNA含量;統計學分析其與患者臨床分期的關繫。結果鼻嚥癌患者外週血淋巴細胞中EB病毒DNA檢齣率為82.76%(72/87),EB病毒DNA拷貝數的中位數為7.58×106拷貝數/ml,Ⅰ、Ⅱ、Ⅲ和Ⅳ期患者每毫升DNA拷貝數分彆為(4.25±1.14)×103、(4.56±0.68)×104、(7.88±1.65)×105和(8.09±1.65)×106;EB病毒DNA載量隨著疾病臨床分期的增加呈現逐步升高的趨勢,且差異顯著(P<0.05)。結論鼻嚥癌患者外週血淋巴細胞中EB病毒DNA載量與患者臨床分期相關,提示EB病毒DNA載量與鼻嚥癌病情進展關繫密切,在臨床上可作為鼻嚥癌患者病情鑑測及判斷療效的一種有效手段。
목적:검측비인암환자외주혈림파세포중EB병독DNA재량,병분석기여비인암림상분기적관계。방법선취2012년1월지2013년1월아원종류과수치주원적87례비인암환자,채용실시형광정량PCR법검측외주혈림파세포EB병독DNA함량;통계학분석기여환자림상분기적관계。결과비인암환자외주혈림파세포중EB병독DNA검출솔위82.76%(72/87),EB병독DNA고패수적중위수위7.58×106고패수/ml,Ⅰ、Ⅱ、Ⅲ화Ⅳ기환자매호승DNA고패수분별위(4.25±1.14)×103、(4.56±0.68)×104、(7.88±1.65)×105화(8.09±1.65)×106;EB병독DNA재량수착질병림상분기적증가정현축보승고적추세,차차이현저(P<0.05)。결론비인암환자외주혈림파세포중EB병독DNA재량여환자림상분기상관,제시EB병독DNA재량여비인암병정진전관계밀절,재림상상가작위비인암환자병정감측급판단료효적일충유효수단。
Objective To detect Epstein-Barr virus DNA load in peripheral blood lymphocytes of nasopharyngeal carcinoma (NPC) patients, and analyze its relationship with the clinical stage of NPC. Methods Real-time quantitative PCR was used to detect EB virus DNA in peripheral blood lymphocytes of 87 NPC patients in our hospital from January 2012 to January 2013, and relationship between the DNA load and NPC clinical stage was analyzed. Results The positive rate of EBV-DNA in NPC patients was 82.76%(72/87) , and a median level of EBV-DNA was 7.58×106copies/ml. The copy numbers of EBV-DNA for stage I, Ⅱ,ⅢandⅣof NPC were (4.25±1.14)×103, (4.56±0.68)×104, (7.88±1.65)×105, and (8.09±1.65)×106, respectively, showing a gradually in-creased tendency from stage I to stage Ⅳ, and the difference was significant (P<0.05). Conclusion EBV-DNA load in peripheral blood lymphocytes is positively correlated with the clinical stage of patients with NPC ,suggesting that EBV-DNA load is closely related to NPC progress, and it can be used as an effective indicator of disease monitoring and efficacy judgement.