浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
1期
17-21
,共5页
赵文胜%石慧%周旭文%房军帆%方剑乔
趙文勝%石慧%週旭文%房軍帆%方劍喬
조문성%석혜%주욱문%방군범%방검교
温经通痹汤%坐骨神经损伤%痛阈%NR2B
溫經通痺湯%坐骨神經損傷%痛閾%NR2B
온경통비탕%좌골신경손상%통역%NR2B
Wen Jing Tong Bi Decoction%sciatic nerve injury%pain threshold%NR2B
[目的]研究温经通痹汤对坐骨神经损伤大鼠痛阈和脊髓背角NR2B表达的影响。[方法]建立坐骨神经损伤大鼠模型，将100只大鼠随机分为模型组（50只）和温经通痹汤组（50只），在造模前及造模后第1、3、7、15、30d时，测定两组大鼠的缩腿阈（Paw Withdrawal Thresholds, PWTs）作为痛阈。造模后对温经通痹汤组大鼠采用温经通痹汤灌胃2次/d、10mL·kg-1/次治疗，在造模后第1、3、7、15、30d等时间点各处死大鼠10只，取腰段脊髓做脊髓背角NR2B受体测定，并进行分析。[结果]两组大鼠造模后第1d的PWTs明显低于造模前的PWTs值（P<0.01），显示造模成功。模型组大鼠造模后第7、15、30d的PWTs值分别为16.28±0.49g、18.21±0.38g和20.05±0.59g，明显高于造模后第1d的13.97±0.44g，差异有统计学意义（P<0.05，P<0.01）。温经通痹汤组大鼠造模后第7、15、30d的PWTs值分别为18.64±0.98g、22.32±1.61g、19.94±0.40g，均高于造模后第1d的13.69±0.58g，差异有统计学意义（P<0.01）。两组间比较，造模后第7、15d时，温经通痹汤组大鼠PWTs值均高于模型组大鼠的PWTs值，差异有统计学意义（P<0.05）。与造模后1d时比较，模型大鼠脊髓背角NR2B的亚基表达在术后第3、7、15、30d差异有统计学意义（P<0.01）；温经通痹汤组脊髓背角NR2B的亚基表达只有在造模后第7、15、30d有非常显著性差异（P<0.01）。两组组间比较，温经通痹汤组脊髓背角NR2B的表达在3、7、15 d显著低于对照组，差异有统计学意义（P<0.01），提示温经通痹汤治疗对CCI模型大鼠脊髓背角的NR2B亚基表达有抑制作用。[结论]温经通痹汤能有效提高坐骨神经损伤大鼠（CCI模型）的痛阈，并在一定治疗时间内存在镇痛累积效应。温经通痹汤在早期较好地抑制脊髓背角NR2B的表达，与改善痛阈的时点吻合，初步说明温经通痹汤提高痛阈与调节脊髓背角NR2B亚基的表达有关。
[目的]研究溫經通痺湯對坐骨神經損傷大鼠痛閾和脊髓揹角NR2B錶達的影響。[方法]建立坐骨神經損傷大鼠模型，將100隻大鼠隨機分為模型組（50隻）和溫經通痺湯組（50隻），在造模前及造模後第1、3、7、15、30d時，測定兩組大鼠的縮腿閾（Paw Withdrawal Thresholds, PWTs）作為痛閾。造模後對溫經通痺湯組大鼠採用溫經通痺湯灌胃2次/d、10mL·kg-1/次治療，在造模後第1、3、7、15、30d等時間點各處死大鼠10隻，取腰段脊髓做脊髓揹角NR2B受體測定，併進行分析。[結果]兩組大鼠造模後第1d的PWTs明顯低于造模前的PWTs值（P<0.01），顯示造模成功。模型組大鼠造模後第7、15、30d的PWTs值分彆為16.28±0.49g、18.21±0.38g和20.05±0.59g，明顯高于造模後第1d的13.97±0.44g，差異有統計學意義（P<0.05，P<0.01）。溫經通痺湯組大鼠造模後第7、15、30d的PWTs值分彆為18.64±0.98g、22.32±1.61g、19.94±0.40g，均高于造模後第1d的13.69±0.58g，差異有統計學意義（P<0.01）。兩組間比較，造模後第7、15d時，溫經通痺湯組大鼠PWTs值均高于模型組大鼠的PWTs值，差異有統計學意義（P<0.05）。與造模後1d時比較，模型大鼠脊髓揹角NR2B的亞基錶達在術後第3、7、15、30d差異有統計學意義（P<0.01）；溫經通痺湯組脊髓揹角NR2B的亞基錶達隻有在造模後第7、15、30d有非常顯著性差異（P<0.01）。兩組組間比較，溫經通痺湯組脊髓揹角NR2B的錶達在3、7、15 d顯著低于對照組，差異有統計學意義（P<0.01），提示溫經通痺湯治療對CCI模型大鼠脊髓揹角的NR2B亞基錶達有抑製作用。[結論]溫經通痺湯能有效提高坐骨神經損傷大鼠（CCI模型）的痛閾，併在一定治療時間內存在鎮痛纍積效應。溫經通痺湯在早期較好地抑製脊髓揹角NR2B的錶達，與改善痛閾的時點吻閤，初步說明溫經通痺湯提高痛閾與調節脊髓揹角NR2B亞基的錶達有關。
[목적]연구온경통비탕대좌골신경손상대서통역화척수배각NR2B표체적영향。[방법]건립좌골신경손상대서모형，장100지대서수궤분위모형조（50지）화온경통비탕조（50지），재조모전급조모후제1、3、7、15、30d시，측정량조대서적축퇴역（Paw Withdrawal Thresholds, PWTs）작위통역。조모후대온경통비탕조대서채용온경통비탕관위2차/d、10mL·kg-1/차치료，재조모후제1、3、7、15、30d등시간점각처사대서10지，취요단척수주척수배각NR2B수체측정，병진행분석。[결과]량조대서조모후제1d적PWTs명현저우조모전적PWTs치（P<0.01），현시조모성공。모형조대서조모후제7、15、30d적PWTs치분별위16.28±0.49g、18.21±0.38g화20.05±0.59g，명현고우조모후제1d적13.97±0.44g，차이유통계학의의（P<0.05，P<0.01）。온경통비탕조대서조모후제7、15、30d적PWTs치분별위18.64±0.98g、22.32±1.61g、19.94±0.40g，균고우조모후제1d적13.69±0.58g，차이유통계학의의（P<0.01）。량조간비교，조모후제7、15d시，온경통비탕조대서PWTs치균고우모형조대서적PWTs치，차이유통계학의의（P<0.05）。여조모후1d시비교，모형대서척수배각NR2B적아기표체재술후제3、7、15、30d차이유통계학의의（P<0.01）；온경통비탕조척수배각NR2B적아기표체지유재조모후제7、15、30d유비상현저성차이（P<0.01）。량조조간비교，온경통비탕조척수배각NR2B적표체재3、7、15 d현저저우대조조，차이유통계학의의（P<0.01），제시온경통비탕치료대CCI모형대서척수배각적NR2B아기표체유억제작용。[결론]온경통비탕능유효제고좌골신경손상대서（CCI모형）적통역，병재일정치료시간내존재진통루적효응。온경통비탕재조기교호지억제척수배각NR2B적표체，여개선통역적시점문합，초보설명온경통비탕제고통역여조절척수배각NR2B아기적표체유관。
[Objective] To study the effect of Wen Jing Tong Bi Decoction on pain threshold of sciatica and expression of NR2B in spinal dorsal horn. [Methods] 100 Spraque-Dawley rats were randomly divided into 2 groups: Model group(n=50) and Wen Jing Tong Bi Decoction group(n=50). The left sciatic nerve of rat was isolated and constricted. After operation, the Wen Jing Tong Bi Decoction group was treated with 10ml·kg-1 twice a day; and the Model group was administered with the same dose of saline. Paw withdrawal thresholds(PWTs) are used as the pain threshold. Al rats were observed PWTs and executed at the pre-operation and the 1st, 3rd, 5th, 7th, 15th, 30th day after operation. The lumbar spinal cord was used to detect the expres-sion of NR2B receptor in spinal dorsal horn.[Results] 1.Post-operation 1 day, the PWTs of rats decreased obviously. So the model of scratic nerve was made successful y. 2.In model group, the PWTs of postoperative 7th, 15th, 30th day were much higher than those in postoperative 1st day(16.28 ±0.49g vs 13.97±0.44g, 18.21±0.38g vs 13.97±0.44g, 20.25±0.59g vs 13.97±0.44g, P<0.05 or P<0.01). It was suggested that the pain releved since operative 7th day. 3.In Wen Jing Tong Bi Decoction group, the PWTs of operative 7th, 15th, 30th day were much higher than those in postoperative 1st day(18.64 ± 0.98g vs 13.69±0.58g, 22.32±1.61g vs 13.69 ±0.58g, 19.94±0.40g vs 13.69±0.58g, P<0.01), and there were significant differences. 4. The PWTs of op-erative 7th and 15th day in Wen Jing Tong Bi Decoction group were much higher than those in model group( P<0.05).There was no difference in the pain threshold of operative 30th days between two groups( P>0.05), and the pain threshold in postoperative 30 days had no significant difference compared with that in preoperative(P>0.05). 5.The result of NR2B in spinal dorsal horn: there were significant differences in the actual staining gray scale at any time of post-operation. In Wen Jing Tong Bi Decoction group, there were significant differences in the actual staining gray scale at post-operation 7th day, 15th day and 30 days than that at pre-operation(P<0.01). The result of NR2B at operative 3rd, 7th and 15th day in Wen Jing Tong Bi Decoction group was much lower than those in model group(P<0.01).[Conclusion] Wen Jing Tong Bi Decoction could relieve the pain of rats; increase the pain threshold of the chronic constrictive injury(CCI) model and in certain time(about half a month) have a good analgesic cumulative effect. And Wen Jing Tong Bi Decoction could inhibit the expression of NR2B, as the same as the improvement of pain threshold point. It suggested that Wen Jing Tong Bi Decoction could increase the pain threshold by adjusting the expression of NR2B. It might be the molecular mechanism of analgesia.
