针灸临床杂志
針灸臨床雜誌
침구림상잡지
JOURNAL OF CLINICAL ACUPUNCTURE AND MOXIBUSTION
2014年
1期
42-45
,共4页
陈素辉%孙华%徐虹%张亚敏%王富明%高扬
陳素輝%孫華%徐虹%張亞敏%王富明%高颺
진소휘%손화%서홍%장아민%왕부명%고양
针刺%百会%足三里%脑缺血再灌注损伤%白细胞介素-6
針刺%百會%足三裏%腦缺血再灌註損傷%白細胞介素-6
침자%백회%족삼리%뇌결혈재관주손상%백세포개소-6
Acupuncture%“Baihui”( GV20 )%“Zusanli”( ST36 )%Cerebral ischemia reperfusion injury%Inter-leukin-6
目的:探讨针刺百会和足三里穴对脑缺血再灌注损伤大鼠双侧脑组织白细胞介素-6的表达变化。方法:采用随机数字表将SD大鼠随机分为针刺组(A)、模型组(M)和假手术组(S)3个大组,各大组再根据大鼠再灌注后时间分为12 h、24 h、48 h、72 h、96 h和144 h共6个时间组,每个时间组有6只大鼠,设正常组( N)6只。模型组及针刺组应用线栓法制备脑缺血再灌注损伤大鼠模型,模型组造模成功后不做治疗干预;针刺组造模成功后,取百会和足三里穴进行针刺治疗20 min,每天1次;假手术组未插入线栓,其余同模型组;正常组未进行处理及治疗。应用免疫组化法检测大鼠双侧脑组织IL-6的表达。结果:IL-6在脑缺血大鼠患侧脑区呈单峰表达,模型组峰值时间为96 h,针刺组为72 h,针刺组表达量显著低于模型组,但高于假手术组和正常组;IL-6在脑缺血大鼠健侧脑区表达高于假手术组和正常组,除12 h和96 h组外,针刺组各个时间点的表达高于模型组( P<0.05)。结论:针刺百会和足三里穴可通过调节脑缺血损伤大鼠双侧脑组织IL-6的表达,干预脑缺血再灌注损伤大鼠的炎症反应。
目的:探討針刺百會和足三裏穴對腦缺血再灌註損傷大鼠雙側腦組織白細胞介素-6的錶達變化。方法:採用隨機數字錶將SD大鼠隨機分為針刺組(A)、模型組(M)和假手術組(S)3箇大組,各大組再根據大鼠再灌註後時間分為12 h、24 h、48 h、72 h、96 h和144 h共6箇時間組,每箇時間組有6隻大鼠,設正常組( N)6隻。模型組及針刺組應用線栓法製備腦缺血再灌註損傷大鼠模型,模型組造模成功後不做治療榦預;針刺組造模成功後,取百會和足三裏穴進行針刺治療20 min,每天1次;假手術組未插入線栓,其餘同模型組;正常組未進行處理及治療。應用免疫組化法檢測大鼠雙側腦組織IL-6的錶達。結果:IL-6在腦缺血大鼠患側腦區呈單峰錶達,模型組峰值時間為96 h,針刺組為72 h,針刺組錶達量顯著低于模型組,但高于假手術組和正常組;IL-6在腦缺血大鼠健側腦區錶達高于假手術組和正常組,除12 h和96 h組外,針刺組各箇時間點的錶達高于模型組( P<0.05)。結論:針刺百會和足三裏穴可通過調節腦缺血損傷大鼠雙側腦組織IL-6的錶達,榦預腦缺血再灌註損傷大鼠的炎癥反應。
목적:탐토침자백회화족삼리혈대뇌결혈재관주손상대서쌍측뇌조직백세포개소-6적표체변화。방법:채용수궤수자표장SD대서수궤분위침자조(A)、모형조(M)화가수술조(S)3개대조,각대조재근거대서재관주후시간분위12 h、24 h、48 h、72 h、96 h화144 h공6개시간조,매개시간조유6지대서,설정상조( N)6지。모형조급침자조응용선전법제비뇌결혈재관주손상대서모형,모형조조모성공후불주치료간예;침자조조모성공후,취백회화족삼리혈진행침자치료20 min,매천1차;가수술조미삽입선전,기여동모형조;정상조미진행처리급치료。응용면역조화법검측대서쌍측뇌조직IL-6적표체。결과:IL-6재뇌결혈대서환측뇌구정단봉표체,모형조봉치시간위96 h,침자조위72 h,침자조표체량현저저우모형조,단고우가수술조화정상조;IL-6재뇌결혈대서건측뇌구표체고우가수술조화정상조,제12 h화96 h조외,침자조각개시간점적표체고우모형조( P<0.05)。결론:침자백회화족삼리혈가통과조절뇌결혈손상대서쌍측뇌조직IL-6적표체,간예뇌결혈재관주손상대서적염증반응。
Objective:To explore the effects of acupuncture of DV 20 and ST36 on the expression of interleukin-6 in rats'brain after cerebral ischemia reperfusion injury ( CIRI) .Methods:CIRI rat models were established by right middle cerebral artery occlusion and reperfusion .SD rats were randomly divided into four groups:acu-puncture group (A), model group (M), sham-operation group (S) and normal control group (N).The Group M didn't do any disposal after successful modeling .The Group A was acupunctured at Baihui ( GV20 ) and Zusanli ( ST36) for 20 min once a day after successful operation .The Group S was to imitate the operation procedure , without inserting line bolt into artery and with no treatment .The Group N was regarded as blank control group , without any operation and treatment .The expression of Interleukin -6 ( IL-6) in bilateral brain tissues was examined by immunohistochemistry .Results:The expression of IL -6 in cerebral ischemia regions exhibited a single-peak, the peak time in CIRI of group M was at 96h, while in group A it was at 72h, com-pared with group M , the expression of IL -6 in group A declined obviously , but the level was still above the group S and group N; the expression of IL -6 in cerebral non -ischemia regions was above the group S and group N, except the time-points of 12h and 96h,and the expression in group A was higher than that in group M.Conclusion:Acupuncture of GV20 and ST36 can affect the expression of IL -6 in bilateral cerebral tissues of CIRI rats, interfering the inflammation response of CIRI .