浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
1期
73-77
,共5页
红腺忍冬叶%HPLC-ELSD法%葡萄糖%果糖
紅腺忍鼕葉%HPLC-ELSD法%葡萄糖%果糖
홍선인동협%HPLC-ELSD법%포도당%과당
leaves ofLonicera hypoglauca Miq.%HPLC-ELSD%glucose%fructose
[目的]建立用HPLC-ELSD法测定红腺忍冬叶提取物中葡萄糖和果糖含量的方法，并应用此方法研究红腺忍冬叶不同溶剂提取物中葡萄糖和果糖含量的变化。[方法]采用Lichrospher NH2色谱柱，乙腈-水(90：10)为流动相，柱温25℃，流速0.8mL/min；ELSD漂移管温度70℃，载气为氮气，流速1.6L/min。[结果]红腺忍冬叶提取物中的葡萄糖和果糖能够得到较好地分离，葡萄糖和果糖的线性范围分别为0.5840~1.168mg·mL-1、0.8256~1.8576mg·mL-1，r分别为0.9994、0.9991；平均加样回收率分别为96.88%、97.55%，RSD分别为1.11%、1.15%；葡萄糖和果糖的稳定性(RSD)分别为2.12%、0.38%，重复性(RSD)分别为1.31%、0.24%。其中，水提物中葡萄糖和果糖的含量最高（分别为2.19mg·mL-1、4.10mg·mL-1），50%醇提物次之（含量分别为1.13mg·mL-1、2.12mg·mL-1），95%醇提物中含量最低（分别为0.45mg·mL-1、0.65mg·mL-1）。[结论]该方法简单、准确、重复性好，可用于测定红腺忍冬叶提取物中葡萄糖和果糖的含量。该批次红腺忍冬叶中，葡萄糖和果糖含量为水提液>50%乙醇提取物>95%乙醇提取物。
[目的]建立用HPLC-ELSD法測定紅腺忍鼕葉提取物中葡萄糖和果糖含量的方法，併應用此方法研究紅腺忍鼕葉不同溶劑提取物中葡萄糖和果糖含量的變化。[方法]採用Lichrospher NH2色譜柱，乙腈-水(90：10)為流動相，柱溫25℃，流速0.8mL/min；ELSD漂移管溫度70℃，載氣為氮氣，流速1.6L/min。[結果]紅腺忍鼕葉提取物中的葡萄糖和果糖能夠得到較好地分離，葡萄糖和果糖的線性範圍分彆為0.5840~1.168mg·mL-1、0.8256~1.8576mg·mL-1，r分彆為0.9994、0.9991；平均加樣迴收率分彆為96.88%、97.55%，RSD分彆為1.11%、1.15%；葡萄糖和果糖的穩定性(RSD)分彆為2.12%、0.38%，重複性(RSD)分彆為1.31%、0.24%。其中，水提物中葡萄糖和果糖的含量最高（分彆為2.19mg·mL-1、4.10mg·mL-1），50%醇提物次之（含量分彆為1.13mg·mL-1、2.12mg·mL-1），95%醇提物中含量最低（分彆為0.45mg·mL-1、0.65mg·mL-1）。[結論]該方法簡單、準確、重複性好，可用于測定紅腺忍鼕葉提取物中葡萄糖和果糖的含量。該批次紅腺忍鼕葉中，葡萄糖和果糖含量為水提液>50%乙醇提取物>95%乙醇提取物。
[목적]건립용HPLC-ELSD법측정홍선인동협제취물중포도당화과당함량적방법，병응용차방법연구홍선인동협불동용제제취물중포도당화과당함량적변화。[방법]채용Lichrospher NH2색보주，을정-수(90：10)위류동상，주온25℃，류속0.8mL/min；ELSD표이관온도70℃，재기위담기，류속1.6L/min。[결과]홍선인동협제취물중적포도당화과당능구득도교호지분리，포도당화과당적선성범위분별위0.5840~1.168mg·mL-1、0.8256~1.8576mg·mL-1，r분별위0.9994、0.9991；평균가양회수솔분별위96.88%、97.55%，RSD분별위1.11%、1.15%；포도당화과당적은정성(RSD)분별위2.12%、0.38%，중복성(RSD)분별위1.31%、0.24%。기중，수제물중포도당화과당적함량최고（분별위2.19mg·mL-1、4.10mg·mL-1），50%순제물차지（함량분별위1.13mg·mL-1、2.12mg·mL-1），95%순제물중함량최저（분별위0.45mg·mL-1、0.65mg·mL-1）。[결론]해방법간단、준학、중복성호，가용우측정홍선인동협제취물중포도당화과당적함량。해비차홍선인동협중，포도당화과당함량위수제액>50%을순제취물>95%을순제취물。
[Objective]To establish a method to determine the contents of glucase and fructose in extracts of leaves of Lonicera hypoglauca Miq. using HPLC-ELSD, and apply this method to study the content change of glucose and fructose in different solvents extracts in leaves of Lonicera hypoglauca Miq. [Methods] The chromatographic separation was achieved on Lichrospher NH2 column using a mobile phase composed of a mixture of acetonitrile and water(90:10) at a flow rate of 0.8mL·min-1 and the column temperature was 25℃.The ELSD drift tube temperature was set at 70℃ and the carrying gas was N2 and the flow rate of the gas was 1.6L·min-1. [Results] Glucose and fructose from extracts of the leaves of Lonicera hypoglauca Miq. could be wel separated, and the linearity ranges of glucose and fructose were shown at the concentration of 0.5840~1.168mg·mL-1 and 0.8256~1.8576mg·mL-1, the average recovery rate of glucose and fructose were 96.88%(RSD=1.11%) and 97.55%(RSD=1.15%), the stability of glucose and fructose was 2.12%and 0.38%, and the repeatability was 1.31% and 0.24%. Among the water extract,50% alcohol extract and 95% alcohol extract,the content of the glucose and fructose in the water extract was highest,which was 2.19mg·mL and 4.10mg·mL-1, the 50% alcohol extract was second, which was 1.13mg·mL-1 and 2.12mg·mL-1, and the 95% alcohol extract was lowest, which was 0.45mg·mL-1 and 0.65mg·mL-1.[Conclusion] The method is simple, accurate and has good repeatability, and can be used to determine the contents of glucose and fructose in the extract of leaves of Lonicera hypoglauca Miq. In this batch of leaves of Lonicera hypoglauca Miq., the contents of fructose and glucose were of water extract>50%ethanol extract>95%ethanol extract.
