CAJ | 학술논문

为用体外试验方法评价猪瘟兔化弱毒活疫苗效力，以猪瘟病毒抗体（兔源）为一抗、荧光素标记羊抗兔IgG 为二抗，建立了CSFV兔化弱毒株的间接免疫荧光检测方法（ IFA）。特异性试验表明，用该IFA方法检测CSFV兔化弱毒株接种的RK细胞为阳性，而检测伪狂犬病毒、猪细小病毒病、牛病毒性腹泻/粘膜病毒接种的RK细胞均为阴性。 CSFV兔化弱毒株和活疫苗的兔体感染量（ RID）用兔体测定，半数组织感染量（ TCID50）用IFA测定，拟合RID与TCID50的线性回归方程，确定1RID/mL＝（ TCID50/0．1mL＋200）/12。
위용체외시험방법평개저온토화약독활역묘효력，이저온병독항체（토원）위일항、형광소표기양항토IgG 위이항，건립료CSFV토화약독주적간접면역형광검측방법（ IFA）。특이성시험표명，용해IFA방법검측CSFV토화약독주접충적RK세포위양성，이검측위광견병독、저세소병독병、우병독성복사/점막병독접충적RK세포균위음성。 CSFV토화약독주화활역묘적토체감염량（ RID）용토체측정，반수조직감염량（ TCID50）용IFA측정，의합RID여TCID50적선성회귀방정，학정1RID/mL＝（ TCID50/0．1mL＋200）/12。
Using the antibody against CSFV (C strain) as the primary antibody and the FITC labeled goat anti-rabbit IgG as the second antibody, an indirect immunofluorescence assay was developed to evaluate the efficacy of live classical swine fever virus vaccine (C strain). The IFA showed positive result in detection RK cells inoculated with CSFV (C strain), but negative results in detection RK cells inoculated with pseudorabies virus, porcine parvovirus and bovine viral diarrhea-mucosal virus. Rabbit infection dose ( RID) tested with rabbits and 50% tissue culture infective dose (TCID50) tested with IFA in CSFV (C strain) and vaccine were both carried out. The linear regression equation fitting the TCID50over RID was conducted, and the result is that 1RID/mL= (TCID50/0.1mL+200)/12.