CAJ | 학술논문

从临床病鸭中分离并鉴定了1型鸭疫里默氏杆菌，并在提取鸭疫里默氏杆菌的基因组DNA后，用Sau3A I酶切，回收大小为0．07～4 kb的片段；将酶切片段与酶切的质粒载体pRSET连接后，电转化大肠杆菌Rosetta，成功构建了1型鸭疫里默氏杆菌的基因组文库，经检测库容量约为40000个。随机筛选30个单菌落，用pRSET通用引物进行PCR鉴定，统计结果显示插入片段的大小93．3％在1～3 kb范围内，成功构建了鸭疫里默氏杆菌基因组文库，为鸭疫里默氏杆菌功能基因的克隆及鉴定奠定了基础。
종림상병압중분리병감정료1형압역리묵씨간균，병재제취압역리묵씨간균적기인조DNA후，용Sau3A I매절，회수대소위0．07～4 kb적편단；장매절편단여매절적질립재체pRSET련접후，전전화대장간균Rosetta，성공구건료1형압역리묵씨간균적기인조문고，경검측고용량약위40000개。수궤사선30개단균락，용pRSET통용인물진행PCR감정，통계결과현시삽입편단적대소93．3％재1～3 kb범위내，성공구건료압역리묵씨간균기인조문고，위압역리묵씨간균공능기인적극륭급감정전정료기출。
Riemerella anatipestifer infection was probably the most economically important infectious disease of farm ducks worldwide. In this study, one strain of R. anatipestifer serotype 1 was isolated and identified. Then the genomic DNA of R. anatipestifer serotype 1 was extracted and digested with Sau 3A I and 0.07~4 kb fragments were recovered. The digested bacterial DNA fragments was ligated to the digested pRSET and transformed into competent Escherichia coli Rosetta. Finally, high quality genomic DNA library of R. anatipestifer serotype 1 was succeeded to be constructed, and its content was about 40000, and 93. 3% DNA inserts were from 1 to 3 kb. Further research about functional proteins could be done.