CAJ | 학술논문

根据GenBank已发表的猪轮状病毒VP7基因保守序列，设计特异性引物扩增猪轮状病毒L1株VP 7全基因序列并进行序列测定及分析。结果显示， L1株猪轮状病毒VP 7基因全长为1062 bp，包含一个982 bp的开放阅读框，编码326个氨基酸，与G5型参考毒株核苷酸同源性和推导的氨基酸同源性分别为88．8％～93．7％和93．3％～94．2％，系统进化树分析结果亦显示L1株与G5型参考毒株处于同一个群，由此确定L1株为G5型。与我国近几年流行的G9型毒株NMTL进行抗原表位分析结果显示，两个毒株在 aa25～aa29、aa86～aa102、aa142～aa152、aa211～aa226、aa263～aa286等区域存在明显差异，可能对其免疫保护性存在一定的影响。
근거GenBank이발표적저륜상병독VP7기인보수서렬，설계특이성인물확증저륜상병독L1주VP 7전기인서렬병진행서렬측정급분석。결과현시， L1주저륜상병독VP 7기인전장위1062 bp，포함일개982 bp적개방열독광，편마326개안기산，여G5형삼고독주핵감산동원성화추도적안기산동원성분별위88．8％～93．7％화93．3％～94．2％，계통진화수분석결과역현시L1주여G5형삼고독주처우동일개군，유차학정L1주위G5형。여아국근궤년류행적G9형독주NMTL진행항원표위분석결과현시，량개독주재 aa25～aa29、aa86～aa102、aa142～aa152、aa211～aa226、aa263～aa286등구역존재명현차이，가능대기면역보호성존재일정적영향。
The VP 7 gene ofporcine rotavirus have been cloned and sequenced using a pair of primers designed according to the published cDNA sequence.The results demonstrated that the VP7 gene was 1062 nucleotides long and contains a long open reading frame,which codes for a VP7 protein of 326 amino acids.The VP7 gene shared from 88.8% to 93.7% nucleic acid identity and from 93.3% to 94.2% amino acid identity with PRV G5 genotype reference strains. Phylogenetic analysis revealed that the VP7 gene of L1 strain grouped very closely G5 genotype reference strains. So L1 strain was proved to belong to G5 genotype. Results from the analysis of antigenic index, L1 strain and G9 genotype NMTL strain had differences in amino acid 25 to 29,86 to 102,142 to 152,211 to 226, 263 to 286,which fields would play a important role of immunogenicity of PRV.