中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2014年
1期
46-50
,共5页
肠缺血再灌%清营泻瘀方%力肽%肠屏障功能
腸缺血再灌%清營瀉瘀方%力肽%腸屏障功能
장결혈재관%청영사어방%력태%장병장공능
Intestine ischemia-reperfusion injury%QingYingXieYu(清营泻瘀)Fang%glutamine%gut barrier function
目的：探讨清营泻瘀方联合力肽对肠缺血再灌注损伤大鼠肠屏障的作用及可能机制。方法：SD大鼠72只，随机分为假手术组8只、模型组、中药组（清营泻瘀方）、西药组(力肽)、合用组(清营泻瘀方联合力肽)各16只，后4组按造模后处死时间再分别分为术后2 h组和术后24 h组。采用夹闭肠系膜上动脉45 min的方法诱导肠缺血再灌注损伤，分别观察再灌注后2 h和24 h肠黏膜的病理改变以及血清中TNF-α、IL-10和D-乳酸的水平。结果：清营泻瘀方和力肽都可以减轻肠黏膜的损伤，降低血中IL-10和D-乳酸水平（P＜0.05），但清营泻瘀方在再灌注24 h降低IL-10的水平方面优于力肽（P＜0.05），清营泻瘀方联合力肽在保护肠黏膜和降低血D-乳酸的水平方面均优于单纯使用清营泻瘀方或力肽（P＜0.05）。结论：清营泻瘀方联合力肽对肠缺血再灌注大鼠肠屏障有显著的保护作用，其可能机制与荡涤肠胃宿垢，减少细菌移位，调节炎症介质平衡有关。
目的：探討清營瀉瘀方聯閤力肽對腸缺血再灌註損傷大鼠腸屏障的作用及可能機製。方法：SD大鼠72隻，隨機分為假手術組8隻、模型組、中藥組（清營瀉瘀方）、西藥組(力肽)、閤用組(清營瀉瘀方聯閤力肽)各16隻，後4組按造模後處死時間再分彆分為術後2 h組和術後24 h組。採用夾閉腸繫膜上動脈45 min的方法誘導腸缺血再灌註損傷，分彆觀察再灌註後2 h和24 h腸黏膜的病理改變以及血清中TNF-α、IL-10和D-乳痠的水平。結果：清營瀉瘀方和力肽都可以減輕腸黏膜的損傷，降低血中IL-10和D-乳痠水平（P＜0.05），但清營瀉瘀方在再灌註24 h降低IL-10的水平方麵優于力肽（P＜0.05），清營瀉瘀方聯閤力肽在保護腸黏膜和降低血D-乳痠的水平方麵均優于單純使用清營瀉瘀方或力肽（P＜0.05）。結論：清營瀉瘀方聯閤力肽對腸缺血再灌註大鼠腸屏障有顯著的保護作用，其可能機製與盪滌腸胃宿垢，減少細菌移位，調節炎癥介質平衡有關。
목적：탐토청영사어방연합력태대장결혈재관주손상대서장병장적작용급가능궤제。방법：SD대서72지，수궤분위가수술조8지、모형조、중약조（청영사어방）、서약조(력태)、합용조(청영사어방연합력태)각16지，후4조안조모후처사시간재분별분위술후2 h조화술후24 h조。채용협폐장계막상동맥45 min적방법유도장결혈재관주손상，분별관찰재관주후2 h화24 h장점막적병리개변이급혈청중TNF-α、IL-10화D-유산적수평。결과：청영사어방화력태도가이감경장점막적손상，강저혈중IL-10화D-유산수평（P＜0.05），단청영사어방재재관주24 h강저IL-10적수평방면우우력태（P＜0.05），청영사어방연합력태재보호장점막화강저혈D-유산적수평방면균우우단순사용청영사어방혹력태（P＜0.05）。결론：청영사어방연합력태대장결혈재관주대서장병장유현저적보호작용，기가능궤제여탕조장위숙구，감소세균이위，조절염증개질평형유관。
Objective To study the effects and mechanisms of QingYingXieYu(清营泻瘀)Fang (QXF) to-gether with glutamine in the protection of gut barrier function of intestinal ischemia-reperfusion in rats. Methods Seventy two SD rats were randomly divided into normal group(n=8), model group (n=16), QXF treat-ment group (n=16), glutamine group (n=16), QXF combining glutamine treatment group (n=16).The last four groups were also randomly divided into two equal groups after reperfusion at 2 hour and 24 hour respectively. The pathologic injury of the intestines and the levels of TNF-α,IL-10 and D lactic acid in blood were examined at 2 hours point and 24 hours point after reperfusion respectively.The statistical analysis was finished with SPSS11.0 software. Results The use of QXF and glutamine alone both alleviated the pathologic injury of the intestines, and reduced the levels of IL-10 and D lactic acid. The use of QXF was better than glutamine in re-ducing the level of IL-10. QXF combined with glutamine presented better curative effects than QXF and gluta-mine alone. Conclusion QXF together with glutamine can greatly reduce the gut injury of intestinal ischemia reperfusion with the following possible mechanism:to clear up dunghill in intestine,to regulate the balance of in-flammatory mediators and to protect gut barrier function.
