CAJ | 학술논문

背景与目的：NK/T细胞淋巴瘤(natural killer/T cell lymphoma)疗效差，多药耐药(multidrug resistance，MDR)的产生是引起肿瘤产生耐药和化疗疗效降低及失败的原因之一，本文通过研究多药耐药相关蛋白4(ABCC4 multidrug resistance associated protein，ABCC4/MRP)基因和多药耐药相关蛋白5(ABCC5 multidrug resistance associated protein，ABCC5/MRP)基因在NK/T细胞淋巴瘤SNK-6、YTS细胞株和组织中的表达情况，结合临床疗效了解其与预后的关系。方法：应用实时荧光定量PCR(real-time PCR)技术和免疫组织化学法(immunohistochemistry，IHC)检测ABCC4/MRP4、ABCC5/MRP5基因mRNA和蛋白表达水平。结果：与正常NK细胞相比，ABCC4/MRP4、ABCC5/MRP5基因在SNK-6、YTS细胞株中高表达(P<0.05)；与鼻炎组织相比，ABCC4/MRP4、ABCC5/MRP5基因在NK/T细胞淋巴瘤患者组织中高表达(P<0.05)；ABCC4/MRP4、ABCC5/MRP5基因表达量的高低和临床疗效呈负相关(P<0.05)。结论：ABCC4/MRP4、ABCC5/MRP5基因及其表达蛋白影响NK/T细胞淋巴瘤的疗效。
배경여목적：NK/T세포림파류(natural killer/T cell lymphoma)료효차，다약내약(multidrug resistance，MDR)적산생시인기종류산생내약화화료료효강저급실패적원인지일，본문통과연구다약내약상관단백4(ABCC4 multidrug resistance associated protein，ABCC4/MRP)기인화다약내약상관단백5(ABCC5 multidrug resistance associated protein，ABCC5/MRP)기인재NK/T세포림파류SNK-6、YTS세포주화조직중적표체정황，결합림상료효료해기여예후적관계。방법：응용실시형광정량PCR(real-time PCR)기술화면역조직화학법(immunohistochemistry，IHC)검측ABCC4/MRP4、ABCC5/MRP5기인mRNA화단백표체수평。결과：여정상NK세포상비，ABCC4/MRP4、ABCC5/MRP5기인재SNK-6、YTS세포주중고표체(P<0.05)；여비염조직상비，ABCC4/MRP4、ABCC5/MRP5기인재NK/T세포림파류환자조직중고표체(P<0.05)；ABCC4/MRP4、ABCC5/MRP5기인표체량적고저화림상료효정부상관(P<0.05)。결론：ABCC4/MRP4、ABCC5/MRP5기인급기표체단백영향NK/T세포림파류적료효。
Background and purpose: Natural killer/T cell lymphoma in poor effects, the production of multidrug resistance is one of the reasons to reduce the chemotherapy effect or failure. This study aimed to discuss the multidrug resistance associated protein 4 (ABCC4/MRP4) gene and multidrug resistance associated protein 5 (ABCC5/MRP5) gene expression in NK/T cell lymphoma SNK-6, YTS cell lines and NK/T cell lymphoma tissues, and the relationship between the level of ABCC4/MRP4, ABCC5/MRP5 gene expression and the clinical efifcacy. Methods:Real-time lfuorescence quantitative PCR (Real time-PCR) and immunohistochemical method (IHC) were used to detect the ABCC4/MRP4, ABCC5/MRP5 gene and protein expression. Results:Compared with the normal NK cells, ABCC4/MRP4 and ABCC5/MRP5 gene in SNK-6, YTS cell lines were highly expressed (P<0.05); Compared with rhinitis tissues, the expression of ABCC4/MRP4, ABCC5/MRP5 gene was higher in the NK/T cell lymphoma tissues (P<0.05);The expression level of ABCC4/MRP4 and ABCC5/MRP5 gene was negative correlation with clinical efifcacy (P<0.05). Conclusion: The expression of ABCC4/MRP4 and ABCC5/MRP5 gene affects the of clinical efifcacy of NK/T cell lymphoma.