食品与药品
食品與藥品
식품여약품
FOOD AND DRUG
2014年
1期
37-38,39
,共3页
陈慧慧%牛伟霞%李士栋%张淹%赵红燕%郑海荣
陳慧慧%牛偉霞%李士棟%張淹%趙紅燕%鄭海榮
진혜혜%우위하%리사동%장엄%조홍연%정해영
党参%党参炔苷%高效液相色谱法
黨參%黨參炔苷%高效液相色譜法
당삼%당삼결감%고효액상색보법
Codonopsis Radix%lobetyolin%HPLC
目的：测定不同产地党参中党参炔苷的含量，为建立党参的质量标准提供参考。方法采用HPLC，Dionex Acclaim C18色谱柱（4.6 mm×250 mm，5μm）；乙腈-水（22：78）为流动相；检测波长267 nm；柱温为30℃。结果在此色谱条件下，党参炔苷分离度良好，11批不同产地的党参中党参炔苷的含量范围为14.7～158.6μg/g。结论此法操作简便易行，结果稳定可靠，可用于评价党参的质量。
目的：測定不同產地黨參中黨參炔苷的含量，為建立黨參的質量標準提供參攷。方法採用HPLC，Dionex Acclaim C18色譜柱（4.6 mm×250 mm，5μm）；乙腈-水（22：78）為流動相；檢測波長267 nm；柱溫為30℃。結果在此色譜條件下，黨參炔苷分離度良好，11批不同產地的黨參中黨參炔苷的含量範圍為14.7～158.6μg/g。結論此法操作簡便易行，結果穩定可靠，可用于評價黨參的質量。
목적：측정불동산지당삼중당삼결감적함량，위건립당삼적질량표준제공삼고。방법채용HPLC，Dionex Acclaim C18색보주（4.6 mm×250 mm，5μm）；을정-수（22：78）위류동상；검측파장267 nm；주온위30℃。결과재차색보조건하，당삼결감분리도량호，11비불동산지적당삼중당삼결감적함량범위위14.7～158.6μg/g。결론차법조작간편역행，결과은정가고，가용우평개당삼적질량。
Objective To determine the content of lobetyolin in Codonopsis Radix from different habitats by HPLC for establishing quality standard. Methods A Dionex Acclaim C18 Column(4.6 mm×250 mm, 5μm)was used with acetonitrile-water(22:78)as the mobile phase and UV detection was at 267 nm under 30℃ Column temperature. Results Under the selected conditions, the lobetyolin peak in chromatograms was completely separated from other peaks. 11 batches of Codonopsis Radix from different habitats were analyzed. The content of lobetyolin ranged from 14.7-158.6 μg/g. Conclusion The method can be simple, reproducible and reliable. It can be used to control the quality of Codonopsis Radix.
