CAJ | 학술논문

制备了硫化银-多壁碳纳米管( Ag2 S-MWNTs)纳米复合材料,构置了Mb-Ag2 S-MWNTs-CHIT/GCE,并研究了肌红蛋白( Mb)在该修饰电极上的直接电化学和电催化行为。采用扫描电镜和透射电镜表征了Ag2 S-MWNTs的形貌,利用循环伏安法对Mb的电化学行为进行研究。 Ag2 S能够均一、稳定的在MWNTs表面生长,所构置的修饰电极在PBS中出现一对峰形良好的、准可逆的氧化还原峰,并对过氧化氢( H2 O2)表现出良好的电催化作用,测定H2O2的线性范围为1．0×10-6~2．5×10-4 mol·L-1,检出限为3×10-7 mol·L-1(S/N=3)。 Ag2 S-MWNTs纳米复合材料能显著提高氧化还原蛋白质(酶)的直接电子传递速率,所构置的修饰电极可为制备基于蛋白质(酶)的第三代电化学生物传感器提供一良好的研究平台。
제비료류화은-다벽탄납미관( Ag2 S-MWNTs)납미복합재료,구치료Mb-Ag2 S-MWNTs-CHIT/GCE,병연구료기홍단백( Mb)재해수식전겁상적직접전화학화전최화행위。채용소묘전경화투사전경표정료Ag2 S-MWNTs적형모,이용순배복안법대Mb적전화학행위진행연구。 Ag2 S능구균일、은정적재MWNTs표면생장,소구치적수식전겁재PBS중출현일대봉형량호적、준가역적양화환원봉,병대과양화경( H2 O2)표현출량호적전최화작용,측정H2O2적선성범위위1．0×10-6~2．5×10-4 mol·L-1,검출한위3×10-7 mol·L-1(S/N=3)。 Ag2 S-MWNTs납미복합재료능현저제고양화환원단백질(매)적직접전자전체속솔,소구치적수식전겁가위제비기우단백질(매)적제삼대전화학생물전감기제공일량호적연구평태。
Ag2 S-MWNTs nanoconposites was prepared and then a Mb-Ag2 S-MWNTs-CHIT/GCE was fabricated. Based on the fabri-cated Mb-Ag2 S-MWNTs-CHIT/GCE,the direct electrochemistry and electrocatalytic properties of the immobilized Mb were investi-gated. Scanning electron microscopic and Transmission electron microscopic were used to characterize the morphology of the Ag2 S-MWNTs. Cyclic voltammetry and amperometry were used for studying the electrochemical behaviors of Mb. Uniform and stable Ag2 S was growing at the MWNTs surface,and the fabricated electrode showed a well-defined and quasi-reversible redox peaks and good electrocatalytic ability for hydrogen peroxide( H2 O2 ) reduction. The resulting modified electrode could detect H2 O2 in a linear range of 1. 0 × 10-6 ~2. 5 × 10-4 mol·L-1 and the detection limit was 3 × 10-7 mol·L-1 at a signal-to-noise ratio of 3. Ag2 S-MWNTs in-creased the direct electron transfer rate dramatically and the modified electrode can provide a good platform for the investigation of the third generation biosensors.