化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2014年
1期
33-37
,共5页
5-FU%牛血清白蛋白%荧光猝灭%紫外光谱%红外光谱
5-FU%牛血清白蛋白%熒光猝滅%紫外光譜%紅外光譜
5-FU%우혈청백단백%형광졸멸%자외광보%홍외광보
5-FU%bovine albumin serum%fluorescence quenching%UV spectra%IR spectroscopy
采用荧光、紫外和红外光谱法研究了5-氟尿嘧啶(5-FU)同牛血清白蛋白( BSA)的相互作用。5-FU对BSA的内源荧光具有强烈的猝灭作用。二者之间形成不发荧光的复合物是导致荧光猝灭的主要原因。计算了不同温度下两者的结合常数、结合位点数和相应的热力学参数。根据能量转移理论计算了作用距离。采用同步荧光光谱和红外光谱分析了5-FU对BSA构象的影响。
採用熒光、紫外和紅外光譜法研究瞭5-氟尿嘧啶(5-FU)同牛血清白蛋白( BSA)的相互作用。5-FU對BSA的內源熒光具有彊烈的猝滅作用。二者之間形成不髮熒光的複閤物是導緻熒光猝滅的主要原因。計算瞭不同溫度下兩者的結閤常數、結閤位點數和相應的熱力學參數。根據能量轉移理論計算瞭作用距離。採用同步熒光光譜和紅外光譜分析瞭5-FU對BSA構象的影響。
채용형광、자외화홍외광보법연구료5-불뇨밀정(5-FU)동우혈청백단백( BSA)적상호작용。5-FU대BSA적내원형광구유강렬적졸멸작용。이자지간형성불발형광적복합물시도치형광졸멸적주요원인。계산료불동온도하량자적결합상수、결합위점수화상응적열역학삼수。근거능량전이이론계산료작용거리。채용동보형광광보화홍외광보분석료5-FU대BSA구상적영향。
The binding of 5-fluorouracil(5-FU)to bovine serum albumin(BSA)was investigated by molecular spectroscopic(fluores-cence,ultraviolet and IR absorption) techniques. 5-FU can strongly quench the intrinsic fluorescence of BSA. The major quenching mechanism was suggested as the formation of nonflurorescence complex between 5-FU and BSA. The binding constants,the number of bonding sites and corresponding thermodynamic parameters at different temperature were calculated. Based on the F?rster’s theory of non-radiation energy transfer,the binding distance was obtained. The effect of 5-FU on the conformation of BSA was analyzed u-sing synchronous fluorescence and IR spectroscopy.
