CAJ | 학술논문

研究了微波萃取烟草中游离氨基酸和微波水解烟草蛋白质的样品前处理方法,对微波萃取和微波水解条件进行了优化。微波萃取后的游离氨基酸及微波水解后的氨基酸总量采用氨基酸分析仪测定,蛋白质中的氨基酸用差减法计算。微波萃取氨基酸的最佳条件为：盐酸浓度0．05 mol·L-1,物料比1∶20,微波萃取温度50℃,微波萃取时间10 min,该条件下得到的游离氨基酸萃取率与超声30 min的结果基本一致；微波水解蛋白质的最佳条件为：盐酸浓度6 mol·L-1,物料比1∶100,水解温度140℃,水解时间60 min,与传统水解24 h的结果基本一致。用微波辅助法萃取烟草中的游离氨基酸及水解烟草蛋白质,样品前处理时间大大缩短,分析结果令人满意。
연구료미파췌취연초중유리안기산화미파수해연초단백질적양품전처리방법,대미파췌취화미파수해조건진행료우화。미파췌취후적유리안기산급미파수해후적안기산총량채용안기산분석의측정,단백질중적안기산용차감법계산。미파췌취안기산적최가조건위：염산농도0．05 mol·L-1,물료비1∶20,미파췌취온도50℃,미파췌취시간10 min,해조건하득도적유리안기산췌취솔여초성30 min적결과기본일치；미파수해단백질적최가조건위：염산농도6 mol·L-1,물료비1∶100,수해온도140℃,수해시간60 min,여전통수해24 h적결과기본일치。용미파보조법췌취연초중적유리안기산급수해연초단백질,양품전처리시간대대축단,분석결과령인만의。
An efficient microwave-assisted extraction and hydrolysis method was developed to determine the free and protein amino acids in tobacco. The conditions in the microwave process were optimized. The samples were pretreated with microwave extraction and hydrolysis and determined by amino acid analyzer. The results showed that 10 min irradiation at 50℃ with 0. 05 mol·L-1 HCl led to comparable results in terms of total and individual amino acid recovery as the ultrasonic mehtod requiring at least 30 min. The digestion was performed with 6 mol·L-1 HCl(with 1%phenol)for 1h in a microwave at 140 ℃ and the results were in agreement with those obtained by traditional method(110 ℃,24 h).