CAJ | 학술논문

目的：研究醋酸棉酚（GAA）对人舌鳞癌Tca8113细胞增殖的影响和对人类错配修复基因1（hMLH1）甲基化水平的影响，初步探讨其抗肿瘤机理。方法应用MTT法检测GAA对Tca8113细胞生长的影响；应用巢式甲基化特异性聚合酶链反应（nMSP）检测Tca8113细胞在GAA作用48、72 h后hMLH1基因甲基化状态的改变情况。结果MTT检测显示，作用24~72 h，GAA对Tca8113细胞生长具有抑制作用；nMSP检测显示，以终浓度30、15μmol·L-1的GAA作用于Tca8113细胞48、72 h后，hMLH1基因甲基化条带的平均光度值降低，与未经药物处理组比较差异有统计学意义（P<0.05）。结论 GAA能抑制舌鳞癌细胞的生长，并能降低hMLH1基因的甲基化水平。GAA去甲基化作用可能是其具有抗肿瘤作用的机制之一。
목적：연구작산면분（GAA）대인설린암Tca8113세포증식적영향화대인류착배수복기인1（hMLH1）갑기화수평적영향，초보탐토기항종류궤리。방법응용MTT법검측GAA대Tca8113세포생장적영향；응용소식갑기화특이성취합매련반응（nMSP）검측Tca8113세포재GAA작용48、72 h후hMLH1기인갑기화상태적개변정황。결과MTT검측현시，작용24~72 h，GAA대Tca8113세포생장구유억제작용；nMSP검측현시，이종농도30、15μmol·L-1적GAA작용우Tca8113세포48、72 h후，hMLH1기인갑기화조대적평균광도치강저，여미경약물처리조비교차이유통계학의의（P<0.05）。결론 GAA능억제설린암세포적생장，병능강저hMLH1기인적갑기화수평。GAA거갑기화작용가능시기구유항종류작용적궤제지일。
Objective This paper aims to study the effects of gossypol acetic acid (GAA) on proliferation and methylation level of human MutL homologue 1 (hMLH1) gene in human tongue cancer cell line Tca8113. Methods The MTT assay was used to determine the effects of the acid on the proliferation inhibition in Tca8113 cells treated with different GAA concen-trations. Nested methylation-specific polymerase chain reaction (nMSP) was used to detect the change in the methylation level of hMLH1 after 48 and 72 h with 30 and 15μmol·L-1 GAA treatment. Results MTT assay results showed the growth and proliferation inhibition of Tca8113 cells in the experimental GAA group after 24 h to 72 h of GAA treatment. The nMSP results indicated that the average optical density of hMLH1 in the Tca8113 cells significantly changed after the GAA treat-ment (30μmol·L-1 GAA for 48 h and 15μmol·L-1 for 72 h) (P<0.05) compared with that of the control group. Conclusion GAA does not only inhibit Tca8113 proliferation but also has a demethylation effect on the hMLH1 gene. These phenomena may be part of an underlying tumor-suppression mechanism of GAA.