CAJ | 학술논문

目的：研究老年人桩核冠修复前后不同时期根面菌斑中主要致龋菌定植数量变化。方法以单纯随机法选择下颌第一磨牙无咬合功能的患者30人，将一侧需行桩核冠修复的第一磨牙和对侧健康第一磨牙分为受试牙和对照牙。受试牙预备前、预备后72 h、预备后1周及冠修复后1个月，分别采集受试牙与对照牙根面菌斑，然后进行厌氧培养、分离，通过菌落形态学检查、生化特征及聚合酶链反应（PCR）对其中变异链球菌、内氏放线菌和黏性放线菌进行鉴定和菌落计数。结果受试牙变异链球菌预备前、预备后72 h、预备后1周、修复后1个月菌落计数的变化差异有统计学意义（P<0.05）。受试牙黏性放线菌菌落计数在预备前、预备后72 h、预备后1周、修复后1个月的变化差异有统计学意义（P<0.05）。受试牙内氏放线菌菌落计数在预备前、预备后72 h及1周、修复后1月的变化差异有统计学意义（P<0.05）。结论变异链球菌、内氏放线菌及黏性放线菌在预备后1周菌落计数增加，修复后1个月菌落计数降低。以上结果提示在牙体预备后初期，应指导患者进行菌斑控制。
목적：연구노년인장핵관수복전후불동시기근면균반중주요치우균정식수량변화。방법이단순수궤법선택하합제일마아무교합공능적환자30인，장일측수행장핵관수복적제일마아화대측건강제일마아분위수시아화대조아。수시아예비전、예비후72 h、예비후1주급관수복후1개월，분별채집수시아여대조아근면균반，연후진행염양배양、분리，통과균락형태학검사、생화특정급취합매련반응（PCR）대기중변이련구균、내씨방선균화점성방선균진행감정화균락계수。결과수시아변이련구균예비전、예비후72 h、예비후1주、수복후1개월균락계수적변화차이유통계학의의（P<0.05）。수시아점성방선균균락계수재예비전、예비후72 h、예비후1주、수복후1개월적변화차이유통계학의의（P<0.05）。수시아내씨방선균균락계수재예비전、예비후72 h급1주、수복후1월적변화차이유통계학의의（P<0.05）。결론변이련구균、내씨방선균급점성방선균재예비후1주균락계수증가，수복후1개월균락계수강저。이상결과제시재아체예비후초기，응지도환자진행균반공제。
Objective To investigate the changes in the quantity of colonizing Streptococcus mutans(S. mutans) and Acti-nomyces on the root surface plaque before and after post-core crown restoration of the mandibular first molars in the elderly patients. Methods A total of 30 elderly patients, each with one post-core crown restoration of the mandibular first molar, were randomly chosen to participate in the studies. Patients with mandibular first molars with post-core crown restoration and those with healthy contralateral mandibular first molars were divided into the test and control groups, respectively. Root surface plaques of the two groups were collected before tooth preparation, 72 h after preparation, one week after preparation, and one month after restoration. S. mutans, Actinomyces naeslundii (A. naeslundii) and Actinomyces viscosus (A. viscosus), were identified using colony morphology, biochemical techniques, and polymerase chain reaction (PCR). Plaque count was measured using microbial colony count. Results The number of S. mutans and A. viscosus and A. naeslundii in the test group, which was statistically significant (P<0.05), increased 72 h after preparation. The quantities of S. mutans, A. viscosus, and A. naeslundii one week after preparation were significantly different (P<0.05). The plaque count of S. mutans, A. viscosus, and A. naeslundii in the test group decreased one month after restoration (P<0.05). Conclusion The quantities of S. mutans, A. viscosus and A. naeslundii increase one week after preparation but decrease one month after restoration. The finding suggests that dentists should educate patients about plaque control during the early period after tooth preparation.