华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
1期
27-31
,共5页
王彦梅%何家才%李全利%沈际佳
王彥梅%何傢纔%李全利%瀋際佳
왕언매%하가재%리전리%침제가
海藻酸钙%纳米羟磷灰石%复合材料%骨髓基质干细胞%生物相容性
海藻痠鈣%納米羥燐灰石%複閤材料%骨髓基質榦細胞%生物相容性
해조산개%납미간린회석%복합재료%골수기질간세포%생물상용성
sodium alginate%nanohydroxyapatite%com-posite material%bone marrow stromal stem cells%biocom-patibility
目的： 制备海藻酸钙-纳米羟磷灰石复合材料，探讨其作为骨修复材料的可行性。方法 应用化学交联及冷冻干燥技术制备海藻酸钙-纳米羟磷灰石复合材料，通过扫描电镜（SEM）和X光衍射（XRD）对材料性能进行表征，液体置换法测定孔隙率。将第5代骨髓基质干细胞（BMSCs）接种至材料上，倒置显微镜及SEM观察细胞生长情况。材料浸提液与BMSCs共培养，甲基噻唑基四唑（MTT）法检测接种后1、3、5 d的相对增殖率（RGR），并评估毒性分级。新鲜犬血加入材料浸提液进行溶血试验，分光光度计测定光密度值并计算溶血率。结果 制备的海藻酸钙-纳米羟磷灰石复合材料具有多孔性，孔隙率达（88.6±4.5）%。倒置显微镜及SEM观察可见，细胞在材料周围及表面伸展充分、生长旺盛。材料浸提液培养的细胞具有较好的活力，其毒性分级为1级。溶血试验结果表明复合材料的溶血率为1.28%，满足医用生物材料的要求。结论 本研究制备的复合材料具有较高的孔隙率和良好的生物相容性。
目的： 製備海藻痠鈣-納米羥燐灰石複閤材料，探討其作為骨脩複材料的可行性。方法 應用化學交聯及冷凍榦燥技術製備海藻痠鈣-納米羥燐灰石複閤材料，通過掃描電鏡（SEM）和X光衍射（XRD）對材料性能進行錶徵，液體置換法測定孔隙率。將第5代骨髓基質榦細胞（BMSCs）接種至材料上，倒置顯微鏡及SEM觀察細胞生長情況。材料浸提液與BMSCs共培養，甲基噻唑基四唑（MTT）法檢測接種後1、3、5 d的相對增殖率（RGR），併評估毒性分級。新鮮犬血加入材料浸提液進行溶血試驗，分光光度計測定光密度值併計算溶血率。結果 製備的海藻痠鈣-納米羥燐灰石複閤材料具有多孔性，孔隙率達（88.6±4.5）%。倒置顯微鏡及SEM觀察可見，細胞在材料週圍及錶麵伸展充分、生長旺盛。材料浸提液培養的細胞具有較好的活力，其毒性分級為1級。溶血試驗結果錶明複閤材料的溶血率為1.28%，滿足醫用生物材料的要求。結論 本研究製備的複閤材料具有較高的孔隙率和良好的生物相容性。
목적： 제비해조산개-납미간린회석복합재료，탐토기작위골수복재료적가행성。방법 응용화학교련급냉동간조기술제비해조산개-납미간린회석복합재료，통과소묘전경（SEM）화X광연사（XRD）대재료성능진행표정，액체치환법측정공극솔。장제5대골수기질간세포（BMSCs）접충지재료상，도치현미경급SEM관찰세포생장정황。재료침제액여BMSCs공배양，갑기새서기사서（MTT）법검측접충후1、3、5 d적상대증식솔（RGR），병평고독성분급。신선견혈가입재료침제액진행용혈시험，분광광도계측정광밀도치병계산용혈솔。결과 제비적해조산개-납미간린회석복합재료구유다공성，공극솔체（88.6±4.5）%。도치현미경급SEM관찰가견，세포재재료주위급표면신전충분、생장왕성。재료침제액배양적세포구유교호적활력，기독성분급위1급。용혈시험결과표명복합재료적용혈솔위1.28%，만족의용생물재료적요구。결론 본연구제비적복합재료구유교고적공극솔화량호적생물상용성。
Objective To prepare sodium alginate-nanohydroxyapatite composite material and to explore its feasibility as a bone repair material. Methods Sodium alginate-nanohydroxyapatite composite material was prepared using chemical cross-linking and freeze-drying technology. The composite was characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM) and its porosity was measured by liquid displacement method. The fifth passage of bone marrow stromal stem cells (BMSCs) were incubated on the composite material and then growth was observed by inverted micros-cope and SEM. BMSCs were cultured with liquid extracts of the material, methyl thiazolyl tetrazolium (MTT) assay was used to calculate the relative growth rate (RGR) on 1, 3, 5 d and to evaluate the cytotoxicity. Fresh dog blood was added into the liquid extracts to conduct hemolysis test, the spectrophotometer was used to determine the optical density (OD) and to calculate the hemolysis rate. Results Sodium alginate-nanohydroxyapatite composite material displayed porosity, the porous pore rate was (88.6±4.5)%. BMSCs showed full stretching and vigorous growth under inverted microscope and SEM. BMSCs cultured with liquid extracts of the material had good activities. The toxicity of composite material was graded as 1. Hemolysis test results showed that the hemolysis rate of the composite material was 1.28%, thus meeting the requirement of medical biomate-rials. Conclusion The composite material fabricated in this study has high porosity and good biocompatibility.
