CAJ | 학술논문

目的：对流式细胞仪分选获得的p75神经营养蛋白受体（p75NTR）阳性舌鳞状细胞癌细胞进行生物学特性研究。方法通过流式细胞术检测舌鳞状细胞癌细胞系Tca-8113和Cal-27中p75NTR阳性细胞的表达。对流式细胞仪分选获取的p75NTR阳性细胞进行单克隆培养、四甲基偶氮唑盐比色法及划痕实验检测，以未分选细胞为对照，分析p75NTR阳性细胞的生物学特性。结果舌鳞状细胞癌细胞株Tca-8113 、Cal-27中，p75NTR阳性细胞的比例分别为3.1％和1.9％。与未分选的细胞相比，p75NTR阳性细胞的单克隆形成能力更高（Tca-8113细胞，P=0.024；Cal-27细胞，P=0.009）；单克隆p75NTR阳性细胞再培养2周后，p75NTR阳性细胞率分别降为14.5%（Tca-8113）和5.8%（Cal-27）；p75NTR阳性细胞体外增殖能力显著增强，且具有明显的侵袭迁移能力。结论 p75NTR阳性舌鳞状细胞癌细胞具有肿瘤干细胞的特性。
목적： 대류식세포의분선획득적p75신경영양단백수체（p75NTR）양성설린상세포암세포진행생물학특성연구。방법 통과류식세포술검측설린상세포암세포계Tca-8113화Cal-27중p75NTR양성세포적표체。대류식세포의분선획취적p75NTR양성세포진행단극륭배양、사갑기우담서염비색법급화흔실험검측，이미분선세포위대조，분석p75NTR양성세포적생물학특성。결과 설린상세포암세포주Tca-8113 、Cal-27중，p75NTR양성세포적비례분별위3.1％화1.9％。여미분선적세포상비，p75NTR양성세포적단극륭형성능력경고（Tca-8113세포，P=0.024；Cal-27세포，P=0.009）；단극륭p75NTR양성세포재배양2주후，p75NTR양성세포솔분별강위14.5%（Tca-8113）화5.8%（Cal-27）；p75NTR양성세포체외증식능력현저증강，차구유명현적침습천이능력。결론 p75NTR양성설린상세포암세포구유종류간세포적특성。
Objective To study the biological characteristics of p75 neurotrophin receptor positive (p75NTR+) tongue squa-mous cell carcinoma cells which were separated by flow cytometry cell sorting. Methods To determine the biological cha-racteristics of p75NTR+ cells which were separated from Tca-8113 and Cal-27 tongue squamous cell carcinoma cells by flow cytometry cell sorting, including study the capacity of cloning, 3-(4,5)-demethylthiazo(z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, wound healing assay. p75NTR+ cells with non-sorted cells were as control group. Results In Tca-8ll3 and Cal-27 tongue squamous cell carcinoma cell lines, the percentage of p75NTR+ cells were 3.1% and 1.9%. Compared with p75NTR+ cells with non-sorted cells, p75NTR+ cells possess higher capacity of cloning (Tca-8113, P=0.024; Cal-27, P=0.009). The per-centage of p75NTR+ cells of the progeny cells generated from monoclonal p75NTR+ cells decreased to 14.5% (Tca-8113) and 5.8% (Cal-27) after cultured two weeks. p75NTR+ cells possessed higher proliferation ability and higher metastasis ability than non-sorted cells. Conclusion p75NTR+ cells isolated from tongue squamous cell carcinoma have the characteristics of cancer stem cells.