华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
1期
5-8
,共4页
刘西茜%董小倩%张永红%张平%李小玉%陈思秀%冯云
劉西茜%董小倩%張永紅%張平%李小玉%陳思秀%馮雲
류서천%동소천%장영홍%장평%리소옥%진사수%풍운
高迁移率族蛋白N2%移植瘤%Tca8113细胞
高遷移率族蛋白N2%移植瘤%Tca8113細胞
고천이솔족단백N2%이식류%Tca8113세포
high mobility group chromosomal protein N2%transplantation tumor%Tca8113 cell
目的： 研究高迁移率族蛋白N2（HMGN2）对人舌癌裸鼠移植瘤生长的抑瘤作用。方法 裸鼠腋下注射接种舌鳞癌细胞Tca8113以建立裸鼠移植瘤模型，接种7 d成瘤后，设立阴性对照组、HMGN2蛋白组和阳性对照组，在瘤体周边分别注射含washingbuffer Ⅱ、HMGN2蛋白和顺铂的细胞培养基。观察各组裸鼠肿瘤的生长情况，经过4次给药后处死裸鼠，取出瘤块，称其质量计算抑瘤率，并行苏木精-伊红（HE）染色观察形态学变化。结果 成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白组和阳性对照组肿瘤体积明显小于阴性对照组。裸鼠体重在整个实验过程中并没有明显的变化。阴性对照组、HMGN2蛋白组、阳性对照组的肿瘤质量平均值分别为（0.38±0.19）、（0.21±0.15）、（0.23±0.16）g，3组间的差异无统计学意义。HMGN2蛋白组和阳性对照组的抑瘤率分别为45.71%和39.44%。肿瘤经肉眼观察可见阴性对照组瘤块较大，HE染色可见HMGN2蛋白组和阳性对照组细胞坏死明显。结论 HMGN2蛋白可以明显抑制人舌癌裸鼠移植瘤的生长。
目的： 研究高遷移率族蛋白N2（HMGN2）對人舌癌裸鼠移植瘤生長的抑瘤作用。方法 裸鼠腋下註射接種舌鱗癌細胞Tca8113以建立裸鼠移植瘤模型，接種7 d成瘤後，設立陰性對照組、HMGN2蛋白組和暘性對照組，在瘤體週邊分彆註射含washingbuffer Ⅱ、HMGN2蛋白和順鉑的細胞培養基。觀察各組裸鼠腫瘤的生長情況，經過4次給藥後處死裸鼠，取齣瘤塊，稱其質量計算抑瘤率，併行囌木精-伊紅（HE）染色觀察形態學變化。結果 成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白組和暘性對照組腫瘤體積明顯小于陰性對照組。裸鼠體重在整箇實驗過程中併沒有明顯的變化。陰性對照組、HMGN2蛋白組、暘性對照組的腫瘤質量平均值分彆為（0.38±0.19）、（0.21±0.15）、（0.23±0.16）g，3組間的差異無統計學意義。HMGN2蛋白組和暘性對照組的抑瘤率分彆為45.71%和39.44%。腫瘤經肉眼觀察可見陰性對照組瘤塊較大，HE染色可見HMGN2蛋白組和暘性對照組細胞壞死明顯。結論 HMGN2蛋白可以明顯抑製人舌癌裸鼠移植瘤的生長。
목적： 연구고천이솔족단백N2（HMGN2）대인설암라서이식류생장적억류작용。방법 라서액하주사접충설린암세포Tca8113이건립라서이식류모형，접충7 d성류후，설립음성대조조、HMGN2단백조화양성대조조，재류체주변분별주사함washingbuffer Ⅱ、HMGN2단백화순박적세포배양기。관찰각조라서종류적생장정황，경과4차급약후처사라서，취출류괴，칭기질량계산억류솔，병행소목정-이홍（HE）염색관찰형태학변화。결과 성공건립설암라서이식류모형。HMGN2단백조화양성대조조종류체적명현소우음성대조조。라서체중재정개실험과정중병몰유명현적변화。음성대조조、HMGN2단백조、양성대조조적종류질량평균치분별위（0.38±0.19）、（0.21±0.15）、（0.23±0.16）g，3조간적차이무통계학의의。HMGN2단백조화양성대조조적억류솔분별위45.71%화39.44%。종류경육안관찰가견음성대조조류괴교대，HE염색가견HMGN2단백조화양성대조조세포배사명현。결론 HMGN2단백가이명현억제인설암라서이식류적생장。
Objective To evaluate the inhibitory effect of high mobility group chromosomal protein N2 (HMGN2) on human tongue carcinoma tumor in nude mice. Methods A transplantation tumor model in nude mice was constructed by injecting Tca8113 cells. After a week, negative control groups, masculine control groups, and HMGN2 groups were esta-blished. Cell culture of the three groups were separately injected with washing buffer Ⅱ, cis-dichlorodiamineplatinum (DDP), and HMGN2 protein. The tumors were moved after four treatments, and then analyzed by hematoxylin-eosin (HE) staining. Results A transplanted tumor model was established successfully. The volumes of HMGN2 groups and masculine control groups were smaller than those of the negative groups. Mouse weight did not differ among the three groups. Average tumor weight of the negative group was (0.38±0.19) g, that of the HMGN2 group was (0.21±0.15) g, and that of the DDP group was (0.23±0.16) g. These factors indicated no statistically significant difference among the three groups. The tumor inhibitory rate of HMGN2 group was 45.71%, and that of the positive group was 39.44%. Based on evaluation by naked eye, the tumor in the negative group was larger than that in other groups. In addition, cell necrosis was observed during HE staining. Conclusion HMGN2 could significantly inhibit growth of the transplanted tumor in nude mice.
