吉林农业大学学报
吉林農業大學學報
길임농업대학학보
JOURNAL OF JILIN AGRICUL TURAL UNIVERSITY
2014年
1期
30-35
,共6页
袁海滨%刘莹%丁玉骁%王晶%李玉双
袁海濱%劉瑩%丁玉驍%王晶%李玉雙
원해빈%류형%정옥효%왕정%리옥쌍
野艾蒿精油%双斑萤叶甲%熏蒸活性%酶活力
野艾蒿精油%雙斑螢葉甲%熏蒸活性%酶活力
야애호정유%쌍반형협갑%훈증활성%매활력
essential oil of Artemisia lavandulaefolia DC .%Monolepta hieroglyphica (Motschulsky)%fu-migation%enzyme activity
测定了野艾蒿精油对双斑萤叶甲成虫的熏蒸活性及体内几种酶活力的影响。结果表明:野艾蒿精油对双斑萤叶甲成虫具有明显的熏蒸作用,野艾蒿精油剂量为10μL/L,处理8 h后校正死亡率为100%,在12 h后各精油处理组的校正死亡率均>80%。经熏蒸处理24 h后,野艾蒿精油对双斑萤叶甲成虫体内的乙酰胆碱酯酶(AChE)活力抑制作用明显;对双斑萤叶甲成虫体内的α-乙酸萘酯酶(α-NACarE)活力、酸性磷酸酯酶(ACP)活力、碱性磷酸酯酶(ALP)活力均表现出抑制作用。各处理组的谷胱甘肽-S-转移酶(GSTs)活力均高于对照组,野艾蒿精油对双斑萤叶甲成虫体内的谷胱甘肽-S-转移酶(GSTs)活力表现出明显的诱导作用。野艾蒿精油对双斑萤叶甲成虫体内过氧化物酶(POD)活力的影响不明显。
測定瞭野艾蒿精油對雙斑螢葉甲成蟲的熏蒸活性及體內幾種酶活力的影響。結果錶明:野艾蒿精油對雙斑螢葉甲成蟲具有明顯的熏蒸作用,野艾蒿精油劑量為10μL/L,處理8 h後校正死亡率為100%,在12 h後各精油處理組的校正死亡率均>80%。經熏蒸處理24 h後,野艾蒿精油對雙斑螢葉甲成蟲體內的乙酰膽堿酯酶(AChE)活力抑製作用明顯;對雙斑螢葉甲成蟲體內的α-乙痠萘酯酶(α-NACarE)活力、痠性燐痠酯酶(ACP)活力、堿性燐痠酯酶(ALP)活力均錶現齣抑製作用。各處理組的穀胱甘肽-S-轉移酶(GSTs)活力均高于對照組,野艾蒿精油對雙斑螢葉甲成蟲體內的穀胱甘肽-S-轉移酶(GSTs)活力錶現齣明顯的誘導作用。野艾蒿精油對雙斑螢葉甲成蟲體內過氧化物酶(POD)活力的影響不明顯。
측정료야애호정유대쌍반형협갑성충적훈증활성급체내궤충매활력적영향。결과표명:야애호정유대쌍반형협갑성충구유명현적훈증작용,야애호정유제량위10μL/L,처리8 h후교정사망솔위100%,재12 h후각정유처리조적교정사망솔균>80%。경훈증처리24 h후,야애호정유대쌍반형협갑성충체내적을선담감지매(AChE)활력억제작용명현;대쌍반형협갑성충체내적α-을산내지매(α-NACarE)활력、산성린산지매(ACP)활력、감성린산지매(ALP)활력균표현출억제작용。각처리조적곡광감태-S-전이매(GSTs)활력균고우대조조,야애호정유대쌍반형협갑성충체내적곡광감태-S-전이매(GSTs)활력표현출명현적유도작용。야애호정유대쌍반형협갑성충체내과양화물매(POD)활력적영향불명현。
We test the effects of essential oil from Artemigate lavandulaefolia DC .on fumigation activity and several enzymes of Monolepta hieroglyphica (Motschulsky)adults .The result showed that the essen-tial oil from Artemigate lavandulaefolia DC .had obvious fumigation against Monolepta hieroglyphica (Motschulsky)adults,and the corrected mortality was 1 00%when the dose of essential oil from Artemi-gate lavandulaefolia DC .was 1 0μL/L and 8 h after treatment,and the corrected mortality of all the treatment groups reached 80%or more after 1 2 h .Essential oil from Artemigate lavandulaefolia DC .sig-nificantly inhibited AChE activity;the activities ofα-NACarE and ACP and ALP demonstrated inhibition in the body of Monolepta hieroglyphica (Motschulsky)adults .GSTs activity of all treatment groups was higher than that of the control group,and GSTs activity showed obvious induction .POD activity was not obviously affected .