CAJ | 학술논문

目的：研究核因子NF-κB在乙醇诱导小鼠急性肝细胞损伤中的表达及意义。方法：40只小鼠随机分成对照组和3个乙醇(250、500、1000 mmol/kg)染毒组，每组10只。一次性灌胃染毒，16 h后检测小鼠血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)的活力；染毒24 h后采用HE及油红O染色进行病理学观察；采用免疫组织化学法观察小鼠肝细胞中NF-κB蛋白的表达。结果：3个乙醇染毒组小鼠血清中的转氨酶AST和ALT活力均高于对照组(P均<0.05)；HE染色发现小鼠肝组织中出现肝细胞内空泡变性。油红O染色显示损伤的肝细胞内出现大量脂滴。免疫组织化学检测发现500和1000 mmol/kg乙醇染毒组小鼠肝细胞胞质及胞核内NF-κB蛋白表达的积分光密度明显高于对照组(P<0.05)。结论：成功复制了急性小鼠酒精性肝损伤的模型，NF-κB升高是小鼠急性酒精性肝细胞损伤的一个重要分子事件。
목적：연구핵인자NF-κB재을순유도소서급성간세포손상중적표체급의의。방법：40지소서수궤분성대조조화3개을순(250、500、1000 mmol/kg)염독조，매조10지。일차성관위염독，16 h후검측소서혈청중천동안산전안매(AST)、병안산전안매(ALT)적활력；염독24 h후채용HE급유홍O염색진행병이학관찰；채용면역조직화학법관찰소서간세포중NF-κB단백적표체。결과：3개을순염독조소서혈청중적전안매AST화ALT활력균고우대조조(P균<0.05)；HE염색발현소서간조직중출현간세포내공포변성。유홍O염색현시손상적간세포내출현대량지적。면역조직화학검측발현500화1000 mmol/kg을순염독조소서간세포포질급포핵내NF-κB단백표체적적분광밀도명현고우대조조(P<0.05)。결론：성공복제료급성소서주정성간손상적모형，NF-κB승고시소서급성주정성간세포손상적일개중요분자사건。
OBJECTIVE:To investigate the expression and significance of nuclear factor-κappa B in alcohol-induced acute hepatocyte injury. METHODS:Forty mice were randomly divided into a control group and three alcohol treated groups (250,500,1 000 mmol/kg). Each group included ten mice. 16 h after gavage,serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured. After 24 h,the liver was examined by HE staining and oil Red O staining. The expression of NF-κB protein in hepatocytes was assessed by immunohistochemistry. RESULTS:Serum AST and ALT levels in three alcohol-treated groups were significantly increased compared with that in the control group (P<0.05). There was vacuolar degeneration in hepatocytes by HE staining. There were large amounts of Oil Red O staining droplets in the hepatocytes. In alcohol-treated groups (500, 1 000 mmol/kg),the integrated optical density expression of NF-κB protein in hepatocytes cytoplasm and nucleus were significantly higher (P<0.05). CONCLUSION:This study successfully set up an alcohol-induced acute hepatocyte injury mouse model,and illustrated the role of NF-κB in the development and progression of acute alcoholic-induced hepatocyte injury.