CAJ | 학술논문

目的：探讨新疆哈萨克族食管癌患者ALDH1L1基因甲基化状态及其与食管癌发生及预后之间的关系。方法：采用联合亚硫酸氢钠限制性内切酶分析(COBRA)法检测28例哈萨克族食管癌及癌旁组织标本中ALDH1L1基因启动子区甲基化状况，建立Cox回归模型分析临床病理指标、ALDH1L1基因甲基化水平等因素与预后的关系。结果：28例哈族食管癌患者癌组织和癌旁正常组织的ALDH1L1基因启动子甲基化率分别为71.43%和39.28%，差异有统计学意义(P<0.05)。预后因素分析表明肿瘤浸润程度、TNM分期、淋巴结转移、癌组织ALDH1L1基因甲基化水平是影响哈萨克族食管癌预后的独立危险因素。癌组织ALDH1L1基因无甲基化患者术后生存时间长于完全甲基化患者(P<0.05)。结论：癌组织ALDH1L1基因启动子区甲基化状态与新疆哈萨克族食管癌的发生及预后有关。
목적：탐토신강합살극족식관암환자ALDH1L1기인갑기화상태급기여식관암발생급예후지간적관계。방법：채용연합아류산경납한제성내절매분석(COBRA)법검측28례합살극족식관암급암방조직표본중ALDH1L1기인계동자구갑기화상황，건립Cox회귀모형분석림상병리지표、ALDH1L1기인갑기화수평등인소여예후적관계。결과：28례합족식관암환자암조직화암방정상조직적ALDH1L1기인계동자갑기화솔분별위71.43%화39.28%，차이유통계학의의(P<0.05)。예후인소분석표명종류침윤정도、TNM분기、림파결전이、암조직ALDH1L1기인갑기화수평시영향합살극족식관암예후적독립위험인소。암조직ALDH1L1기인무갑기화환자술후생존시간장우완전갑기화환자(P<0.05)。결론：암조직ALDH1L1기인계동자구갑기화상태여신강합살극족식관암적발생급예후유관。
OBJECTIVE:To explore the relationship between the hypermethylation status of ALDH1L1 gene and the occurrence and prognosis of esophageal carcinoma( EC) in Kazakh ethnic groups in Xinjiang.METHODS:The hypermethylation of ALDH1L1 promoter region was evaluated by using combined bisulfite restriction analysis (COBRA) in 28 cases of esophageal cancer tissues from Kazakh patients.Cox model was established to analyze the possible factors influencing the prognosis of EC,including clinicopathological parameters and hypermethylation levels of ALDH1L1 promoter region. RESULTS:The methylation rates of ALDH1L1 promoter region in esophageal cancer tissues and adjacent tissues were 71.43%and 39.28%,respectively. Significant difference were found in the methylation rates of ALDH1L1 promoter region in esophageal cancer and adjacent tissues (P<0.05). Multivariate prognostic analysis showed that the infiltration,TNM stage,lymph node metastasis and hypermethylation levels of ALDH1L1 were independent risk factors. The postoperative survival time of EC patients in Kazakh ethnic with ALDH1L1 gene unmethylation in cancer was significantly longer than those with ALDH1L1 gene methylation (P<0.05). CONCLUSION:Aberrant methylation of ALDH1L1 promoter region may play an important role in the pathogenesis and prognosis of EC in Kazakh ethnic groups.