CAJ | 학술논문

目的：了解玻连蛋白(VTN)对肝肿瘤细胞株SMMC 7721增殖和迁移能力的影响。方法：采用不同浓度VTN包被培养皿后接种、培养细胞，采用激光共聚焦显微镜观察VTN包被后细胞骨架成分微管蛋白形态的变化，用细胞增殖试剂分析VTN对细胞增殖率的影响及分析VTN对凋亡诱导剂抑制增殖作用的影响，采用Transwell侵袭实验检测VTN对细胞迁移能力的影响。结果：激光共聚焦观察结果显示VTN包被有助于促进细胞贴壁及细胞骨架蛋白形态结构的维持；VTN可促进肿瘤细胞的生长且呈剂量效应，并可减弱凋亡诱导剂对细胞增殖的抑制；Transwell侵袭实验结果显示VTN包被可促进细胞迁移。结论：体外实验条件下，VTN有助于肝癌细胞株SMMC 7721的增殖及迁移，提示VTN在肝癌发生过程中可能具有促进肿瘤细胞增殖和迁移的生物学作用。
목적：료해파련단백(VTN)대간종류세포주SMMC 7721증식화천이능력적영향。방법：채용불동농도VTN포피배양명후접충、배양세포，채용격광공취초현미경관찰VTN포피후세포골가성분미관단백형태적변화，용세포증식시제분석VTN대세포증식솔적영향급분석VTN대조망유도제억제증식작용적영향，채용Transwell침습실험검측VTN대세포천이능력적영향。결과：격광공취초관찰결과현시VTN포피유조우촉진세포첩벽급세포골가단백형태결구적유지；VTN가촉진종류세포적생장차정제량효응，병가감약조망유도제대세포증식적억제；Transwell침습실험결과현시VTN포피가촉진세포천이。결론：체외실험조건하，VTN유조우간암세포주SMMC 7721적증식급천이，제시VTN재간암발생과정중가능구유촉진종류세포증식화천이적생물학작용。
OBJECTIVE:To understand the effects of vitronectin (VTN) on cell proliferation and migration in the hepatocellular carcinoma cell line SMMC 7721. METHODS:SMMC 7721 cells were cultured in VTN-coating Petri dishes. The morphology of the cells and the morphology ofβ-tubulin were studied with confocal microscope. WST-1 was used to assess the effect of VTN on cell proliferation rate and the protective effects from the stimuli of apoptosis-inducer. The effect on migration was evaluated with transwell chamber. RESULTS:VTN helped cells adhere to the Petri dishes as well as sustaining the morphology of β-tubulin. VTN could enhance the proliferation rate of SMMC 7721 in a dose-effect mode. VTN could protect the cells from the stimuli of apoptosis-inducer. VTN could also help cell migration across the membrane of transwell chamber. CONCLUSION:VTN might play important roles in the following biological effects,such as sustaining the morphology of the cytoskeleton,promoting adherence of tumor cells,enhancing proliferation,protecting cells from the stimuli of apoptosis-inducer as well as enhancing their migration.