CAJ | 학술논문

目的：探讨脉冲电针和音乐电针治疗阿尔茨海默病（ AD）的可能作用机制，并比较两种电针的疗效差异。方法：将SAMP8小鼠随机分为模型组、脉冲电针组和音乐电针组， SAMR1小鼠作为正常组。两电针组均给予电针百会穴、印堂穴20 min后，点刺水沟穴。治疗结束后，以Morris水迷宫测定小鼠学习记忆能力，实时荧光定量PCR法测定小鼠大脑皮层中APP、ApoE mRNA表达量。结果：与模型组比较，音乐电针组、脉冲电针组逃避潜伏期分别在第4天、第5天明显下降（P＜0．05），目标象限游泳时间均增加（P＜0．05）；脉冲电针组、音乐电针组大脑皮层APP mRNA表达量均显著下降（P＜0．05）。结论：脉冲电针和音乐电针均可改善SAPM8小鼠学习记忆能力；脉冲电针和音乐电针可能通过下调APP mRNA表达发挥抗痴呆作用。
목적：탐토맥충전침화음악전침치료아이자해묵병（ AD）적가능작용궤제，병비교량충전침적료효차이。방법：장SAMP8소서수궤분위모형조、맥충전침조화음악전침조， SAMR1소서작위정상조。량전침조균급여전침백회혈、인당혈20 min후，점자수구혈。치료결속후，이Morris수미궁측정소서학습기억능력，실시형광정량PCR법측정소서대뇌피층중APP、ApoE mRNA표체량。결과：여모형조비교，음악전침조、맥충전침조도피잠복기분별재제4천、제5천명현하강（P＜0．05），목표상한유영시간균증가（P＜0．05）；맥충전침조、음악전침조대뇌피층APP mRNA표체량균현저하강（P＜0．05）。결론：맥충전침화음악전침균가개선SAPM8소서학습기억능력；맥충전침화음악전침가능통과하조APP mRNA표체발휘항치태작용。
Objective:To observe the influence of music electroacupuncture ( EA ) and pulse EA on learning memory ability and APP and ApoE mRNA expression in cerebral cortex of SAMP 8, so as to explore their mech-anisms underlying relief of Alzheimer ’s Disease(AD).Methods:SAMP8 mice were randomly divided into mod-el , pulse EA and music EA groups .SAMR1 mice were used as the control group .EA was applied to “Baihui”(GV20) and“Yintang”(EX-HN3), and then swift pricked “Shuigou”(GV26).The learning memory ability of mice was detected by using Morris Water Maze .APP and ApoE mRNA expression level in cerebral cortex were assayed by fluorescent quantitative real -time PCR.Results:Compared with the model group , the escapel-atency of music EA was decreased considerably on the fourth day ( P<0 .05 ) , but pulse EA was on the fifth day , as well as the level of APP mRNA expression was down -regulated obviously in pulse EA and music EA . Conclusion:Both pulse EA and music EA can effectively improve learning memory ability of SAMP 8 mice which may be closely associated with their effects on down -regulating APP mRNA expression in cerebral cor-tex.