中国药物与临床
中國藥物與臨床
중국약물여림상
CHINESE REMEDIES & CLINICS
2014年
5期
561-563
,共3页
P物质%小神经胶质细胞%脊髓%肿瘤坏死因子α%白细胞介素1
P物質%小神經膠質細胞%脊髓%腫瘤壞死因子α%白細胞介素1
P물질%소신경효질세포%척수%종류배사인자α%백세포개소1
Substance P%Microglia%Spinal cord%Tumor necrosis factor-alpha%Interleukin-1
目的：探讨P物质对脊髓小胶质细胞的作用。方法利用原代培养的脊髓小胶质细胞，P物质孵育后采用免疫组织化学方法观察细胞形态学改变，采用酶联免疫吸附试验（ELISA）方法观察细胞释放肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的情况。结果 P物质孵育后，小胶质细胞表现出激活态的形态学改变，400μmol/L和800μmol/L的P物质孵育6 h后，TNF-α和IL-1β释放增多，12 h时达到高峰，之后逐渐下降。结论 P物质作为脊髓水平传递痛信号的重要神经递质，可以刺激活化脊髓小胶质细胞，促进小胶质细胞合成释放TNF-α和IL-1β，并最终促进慢性痛敏的形成。
目的：探討P物質對脊髓小膠質細胞的作用。方法利用原代培養的脊髓小膠質細胞，P物質孵育後採用免疫組織化學方法觀察細胞形態學改變，採用酶聯免疫吸附試驗（ELISA）方法觀察細胞釋放腫瘤壞死因子-α（TNF-α）和白細胞介素-1β（IL-1β）的情況。結果 P物質孵育後，小膠質細胞錶現齣激活態的形態學改變，400μmol/L和800μmol/L的P物質孵育6 h後，TNF-α和IL-1β釋放增多，12 h時達到高峰，之後逐漸下降。結論 P物質作為脊髓水平傳遞痛信號的重要神經遞質，可以刺激活化脊髓小膠質細胞，促進小膠質細胞閤成釋放TNF-α和IL-1β，併最終促進慢性痛敏的形成。
목적：탐토P물질대척수소효질세포적작용。방법이용원대배양적척수소효질세포，P물질부육후채용면역조직화학방법관찰세포형태학개변，채용매련면역흡부시험（ELISA）방법관찰세포석방종류배사인자-α（TNF-α）화백세포개소-1β（IL-1β）적정황。결과 P물질부육후，소효질세포표현출격활태적형태학개변，400μmol/L화800μmol/L적P물질부육6 h후，TNF-α화IL-1β석방증다，12 h시체도고봉，지후축점하강。결론 P물질작위척수수평전체통신호적중요신경체질，가이자격활화척수소효질세포，촉진소효질세포합성석방TNF-α화IL-1β，병최종촉진만성통민적형성。
Objective To investigate the effect of substance P on the spinal microglia activation. Methods Primary cultured spinal microglia was incubated with substance P. Changes in the morphology of microglia were as-sessed by immunohistochemistry assay. The levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β released from microglia were measured using enzyme-linked immunosorbent assay. Results Incubation with substance P was associated with activation of spinal microglia. Following incubation of 400 μmol/L and 800 μmol/L substance P, there was an increase in the levels of TNF-αand IL-1β, followed by a culmination at 12 hours and a reduction thereafter. Conclusion Substance P, a crucial neurotransmitter of pain in the spinal cord, can activate spinal microglia and in-duce the release of TNF-αand IL-1βleading to the pathogenesis of chronic pain.
