CAJ | 학술논문

目的：探讨人脐带间充质干细胞（hUCMSCs）对食管癌细胞EC9706增殖、迁移的抑制作用和对裸鼠体内移植瘤的抑制作用。方法：体外分离培养hUCMSCs，检测细胞表型；将其和EC9706间接共培养，观察对EC9706增殖、迁移能力的影响。建立动物模型，尾静脉注射hUCMSCs后观察移植瘤生长及转移情况。结果：细胞贴壁9～14 d 后可见成纤维样细胞，流式分析显示第5代细胞均表达 CD29、CD44，不表达 CD34、CD45和HLA-DR。 MTT法及细胞划痕实验显示与对照组相比，实验组EC9706细胞的增殖迁移明显受到抑制，且具有时间依赖性。动物模型对照组和实验组的瘤重分别为（1．83±0．33）、（1．02±0．15）g；平均转移灶为（5．37±1．12）、（1．29±1．05）个，差异有统计学意义。结论：hUCMSCs对EC9706细胞的增殖迁移均有抑制作用，并能抑制移植瘤在裸鼠体内的生长和转移。
목적：탐토인제대간충질간세포（hUCMSCs）대식관암세포EC9706증식、천이적억제작용화대라서체내이식류적억제작용。방법：체외분리배양hUCMSCs，검측세포표형；장기화EC9706간접공배양，관찰대EC9706증식、천이능력적영향。건립동물모형，미정맥주사hUCMSCs후관찰이식류생장급전이정황。결과：세포첩벽9～14 d 후가견성섬유양세포，류식분석현시제5대세포균표체 CD29、CD44，불표체 CD34、CD45화HLA-DR。 MTT법급세포화흔실험현시여대조조상비，실험조EC9706세포적증식천이명현수도억제，차구유시간의뢰성。동물모형대조조화실험조적류중분별위（1．83±0．33）、（1．02±0．15）g；평균전이조위（5．37±1．12）、（1．29±1．05）개，차이유통계학의의。결론：hUCMSCs대EC9706세포적증식천이균유억제작용，병능억제이식류재라서체내적생장화전이。
Objective To investigate the inhibitory effect of human umbilical cord mesenchymal stem cells(hUCMSCs) on proliferation and migration of esophageal cancer EC9706 cells and the transplanted tumor in nudemice. Methods hUCMSCs were isolated and cultured in vitro. The phenotype of hUCMSCs was identified.hUCMSCs were indirectly co-cultured with EC9706 and the effect of co-culture on EC9706 proliferation andmigration was evaluated. Animal models of transplanted tumor were established in nude mice. hUCMSCs wereinfused via the tail vein and the effect of hUCMSCs on the tumor growth and metastasis was investigated. ResultsAt 9 to 14 days after cell adherence, the fibroblast-like cells were observed. Flow cytometry analysis revealed thatCD29 and CD44 were highly expressed on the plasma membrane of the passages 3 cells, with negative expression ofCD34, CD45 and HLA-DR. Results of MTT assay and cell scratch test showed that compared with the control group,the proliferation and migration of EC9706 in the experimental group were significantly inhibited, respectively. Theaverage tumor weight in the control group and in the experimental was (1.83 ± 0.33)g and (1.02 ± 0.15)g,respectively. The average number of metastasis nodules was 5.37 ± 1.12 and 1.29 ± 1.05 respectively, withsignificant difference. Conclusion hUCMSCs can inhibit the proliferation and migration of EC9706 cells andinhibit the growth and metastasis of transplanted tumor in nude mice.