CAJ | 학술논문

目的：建立一系列Rab25表达水平不同的乳腺癌细胞裸鼠移植瘤模型，观察不同水平Rab25表达对乳腺癌裸鼠移植瘤的影响。方法：通过分别上调及沉默Rab25基因在乳腺癌细胞中的表达，建立Rab25表达水平不同的乳腺癌细胞的裸鼠移植瘤模型，测量肿瘤体积，绘制肿瘤生长曲线，检测肿瘤组织中Rab25表达差异。结果：Rab25转染组较干扰组、原株细胞组及空质粒组对裸鼠细胞致瘤能力明显增强。转染组裸鼠瘤体中Rab25 mRNA表达明显增强，而干扰组瘤体Rab25 mRNA表达明显减弱。结论：Rab25为促癌基因，其高表达可直接增强乳腺癌细胞的增殖侵袭能力，而RNA干扰技术有可能为乳腺癌的肿瘤靶向和基因治疗提供新的思路。
목적：건립일계렬Rab25표체수평불동적유선암세포라서이식류모형，관찰불동수평Rab25표체대유선암라서이식류적영향。방법：통과분별상조급침묵Rab25기인재유선암세포중적표체，건립Rab25표체수평불동적유선암세포적라서이식류모형，측량종류체적，회제종류생장곡선，검측종류조직중Rab25표체차이。결과：Rab25전염조교간우조、원주세포조급공질립조대라서세포치류능력명현증강。전염조라서류체중Rab25 mRNA표체명현증강，이간우조류체Rab25 mRNA표체명현감약。결론：Rab25위촉암기인，기고표체가직접증강유선암세포적증식침습능력，이RNA간우기술유가능위유선암적종류파향화기인치료제공신적사로。
Objective To establish nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene , and to observe the effects of Rab25 gene on the growth of implanted tumor. Methods Nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene were constructed through up-regulating or silencing the expression of Rab25 gene in breast cancer cells. The size of implanted tumor was measured, the growth curve of tumor was portrayed, and the expression of Rab25 gene in tumor tissues was detected. Results The tumorigenic effect on nude mice was the strongest and the highest in Rab25 up-regulating group , as compared to those in Rab25 interference group , original cells group, and Rab25 empty plasmid group. The expression of Rab25 mRNA in interference group was significantly reduced. Conclusions Rab25 is a cancer-promoting gene , its high expression could directly enhance the proliferation of breast cancer cells. RNA interference technology had the potential to provide new ideas for tumor targeting and gene therapy for breast cancer.